表达结核抗原的减毒重组李斯特菌的免疫生物学特性

发布时间：2019-01-27 22:42

【摘要】：【目的】结核病是一种由结核分枝杆菌复合群引起的严重危害人类健康的慢性传染病,研制预防结核病的新型疫苗对于有效控制结核病具有重要的公共卫生意义。【方法】本研究对表达M.tb融合蛋白的重组李斯特菌LMΔhly::Ag85b-esat-6的免疫生物学特性进行了初步研究。【结果】实验结果显示,重组菌溶血活性丧失,对C57BL/6小鼠的半数致死剂量提高了4个log值,以0.1 LD50的剂量尾静脉注射C57BL/6小鼠,5天时被完全清除,病理切片结果未显示明显病理变化,表明重组菌具有良好的安全性。以尾静脉途径免疫C57BL/6小鼠,对其诱导的免疫应答测定结果表明,重组菌所运送的结核抗原能诱导小鼠Th1型免疫应答,激发较强的结核抗原特异性的CTL效应。【结论】上述实验结果表明,表达结核分枝杆菌融合蛋白的减毒重组李斯特菌是一种安全的结核病疫苗候选株,具有潜在的应用价值。
[Abstract]:[objective] Tuberculosis is a chronic infectious disease caused by Mycobacterium tuberculosis complex, which is seriously harmful to human health. The development of a novel vaccine for tuberculosis prevention is of great public health significance for the effective control of tuberculosis. [methods] this study was designed to study the immunological activity of recombinant Listeria sp. LM 螖 hly::Ag85b-esat-6 expressing M.tb fusion protein. A preliminary study of the scientific properties has been carried out. [results] the experimental results show that, The hemolytic activity of the recombinant bacteria was lost, and the LD50 of the C57BL/6 mice was increased by 4 log. The mice were completely cleared at the dose of 0.1 LD50 in caudal vein at 5 days. The pathological sections showed no obvious pathological changes. The results showed that the recombinant bacteria had good safety. C57BL/6 mice were immunized by tail vein. The results showed that the tuberculosis antigen transported by recombinant bacteria could induce Th1 type immune response in mice. [conclusion] the above results indicate that the attenuated recombinant Listeria tuberculosis fusion protein is a safe candidate for TB vaccine and has potential application value.
【作者单位】：扬州大学江苏省人兽共患病重点实验室江苏省动物主要疫病防控协同创新中心;
【基金】：国家“973项目”——国家重点基础研究发展计划(2012CB518805) 国家自然科学基金(31101841) 江苏省自然科学基金(BK2011446) 江苏省科技支撑计划(BE2012367)~~
【分类号】：R392

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