非同源脑脊液诱导非同源间充质干细胞分化为神经干细胞的对比研究

发布时间：2019-01-30 09:19

【摘要】：寻找一种符合患者治疗需求的干细胞获取模式,从而进一步推动神经干细胞广泛应用于临床。本实验采用志愿者、患者脑脊液体外诱导异体脐血源间充质干细胞(UMSCs)和患者骨髓源间充质干细胞(BMSCs)分化为神经干细胞,并采用形态学、流式细胞分析、免疫组化、荧光免疫组化法等方法对诱导后的细胞进行签定;同时,记录各诱导组获得临床可用神经干细胞所需的时间及细胞数计数。结果证实各组均可使间充质干细胞(MSCs)定向分化为神经干细胞,所获得的细胞数亦可满足临床需要,但在所需的天数及具体获得细胞数量上各组间有一定差异,其中以患者BMSCs用自愿者脑脊液诱导组所获得神经干细胞计数最高,且其所需时间最短。
[Abstract]:Search for a stem cell acquisition model that meets the needs of patients, and further promote the wide application of neural stem cells in clinical practice. In this experiment, (UMSCs) from cord blood derived mesenchymal stem cells and (BMSCs) from bone marrow derived mesenchymal stem cells were induced to differentiate into neural stem cells from volunteers and cerebrospinal fluid (CSF) in vitro. Morphology, flow cytometry and immunohistochemistry were used. The induced cells were identified by fluorescence immunohistochemical method. At the same time, the time and count of the number of neural stem cells were recorded in each induction group. The results showed that the (MSCs) of mesenchymal stem cells could be differentiated into neural stem cells in each group, and the number of cells obtained could also meet the clinical needs, but there were some differences in the number of specific cells and the number of cells obtained in each group. The number of neural stem cells was the highest in the group of patients with BMSCs induced by cerebrospinal fluid (CSF), and it took the shortest time.
【作者单位】：徐州医学院神经病学教研室;山东省泰安市中医医院脑病中心;徐州医学院附属医院中心实验室;徐州医学院附属医院神经内科;
【分类号】：R329

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