接触性超敏反应小鼠不同部位T细胞亚群的变化

发布时间：2019-03-07 20:13

【摘要】：【目的】比较接触性超敏反应(CHS)小鼠不同部位CD4+/CD8+T细胞以及辅助性T细胞(Th1/Th2)、细胞毒性T细胞(Tc1/Tc2)的平衡状态。【方法】以二硝基氟苯(DNFB)致敏和攻击BALB/c小鼠,建立CHS模型。采用三色流式细胞术分析CHS小鼠脾脏(SP)、耳后淋巴结(RLN)、小肠派氏结(PPs)和肠系膜淋巴结(MLN)中CD4+T细胞和CD8+T细胞的变化及其胞内细胞因子干扰素-γ(IFN-γ)与白细胞介素-4(IL-4)的表达水平,观察CHS小鼠不同部位Th1/Th2、Tc1/Tc2比例的变化。【结果】与正常组比较,CHS组RLN与SP中CD4/CD8比值显著下降(均P0.01),RLN中以Tc1与Th1占优势,SP中以Tc1与Th2占优势,PPs中Tc1细胞比例、CD4/CD8比值显著上升(均P0.05),MLN中各指标无显著性差异。【结论】DNFB诱发的CHS小鼠机体总体免疫平衡向Th1类偏移,Tc1(CD8+IFN-γ+)细胞是主要的效应细胞。
[Abstract]:[objective] to compare CD4 / CD8 T cells and helper T cells (Th1/Th2) in different parts of contact hypersensitive (CHS) mice. [methods] the CHS model was established by sensitizing and attacking BALB/c mice with dinitrofluorobenzene (DNFB). Cytotoxic T cells (Tc1/Tc2) were sensitized and attacked in a balanced state of cytotoxic T cells (CHS). Analysis of (RLN), in (SP), retroauricular lymph nodes of CHS mice by trichromatic flow cytometry The changes of CD4 T cells and CD8 T cells in (PPs) and (MLN) of small intestine and mesenteric lymph nodes, and the expression of interferon-纬 (IFN- 纬) and interleukin-4 (IL-4) in the cells. [results] compared with the normal group, the ratio of CD4/CD8 in RLN and SP in CHS group was significantly decreased (P0.01, Tc1 and Th1 were dominant in), RLN, Tc1 and Th2 were dominant in SP), and the ratio of CD4/CD8 in RLN and SP in CHS group was significantly lower than that in control group (P0.01). The proportion of Tc1 cells and the ratio of CD4/CD8 in PPs were significantly increased (P 0.05), MLN). [conclusion] the total immune balance of CHS mice induced by DNFB shifted to Th1 type. Tc1 (CD8 IFN- 纬) cells are the main effector cells.
【作者单位】：广州中医药大学免疫与分子生物学实验室;无限极(中国)有限公司研发中心;
【基金】：国家自然科学基金项目(编号:30873415) 广东省教育部产学研结合项目(编号:2010A090200041)
【分类号】：R392.8

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