增殖性细胞核抗原、C-fos和Bax蛋白在人胚胎舌组织中的表达

发布时间：2019-03-12 14:11

【摘要】：目的:探讨增殖性细胞核抗原(PCNA)、C-fos和Bax蛋白在人胚胎舌组织不同发育阶段的分布特征。方法:应用免疫组织化学法检测第2~4个月胎龄段共16份人胚胎舌组织内PCNA、C-fos和Bax蛋白的表达,分析其变化规律。结果:第2个月胚龄时,C-fos蛋白在人胚舌组织内未见明显阳性细胞表达分布,PCNA蛋白在人胚舌上皮组织层部分细胞和舌肌组织和纤维组织内少数细胞呈阳性表达。第3个月胎龄时,PCNA和C-fos蛋白在舌上皮组织细胞阳性表达数量最多,舌肌组织和纤维组织内有部分细胞呈阳性表达。第4个月胎龄舌肌组织和纤维组织内PCNA蛋白阳性表达数量增高,C-fos蛋白阳性表达未见明显变化。Bax蛋白仅在第3个月胎龄时呈弱阳性表达。结论:PCNA、C-fos参与调控人胚胎舌组织细胞的分化发育。
[Abstract]:Aim: to investigate the distribution of proliferating cell nuclear antigen (PCNA), C-fos) and Bax protein in human embryonic tongue at different developmental stages. Methods: immunohistochemical method was used to detect the expression of PCNA,C-fos and Bax protein in 16 human fetal tongue tissues at the age of 2 ~ 4 months. Results: at the second month of embryo age, C-fos protein was not expressed in human embryonic tongue tissue, while PCNA protein was expressed in some cells of human embryonic tongue epithelial layer and a few cells in tongue muscle tissue and fibrous tissue. At the third month gestational age, the expression of PCNA and C-fos protein was the highest in the tongue epithelial cells, and some of the cells in the tongue muscle and fibrous tissue were positive. The positive expression of PCNA protein was increased in tongue muscle and fibrous tissue at the fourth month of gestation, while the positive expression of C-fos protein was not significantly changed, but the expression of Bax protein was weakly positive only at the third month of gestation. Conclusion: PCNA,C-fos is involved in the regulation of differentiation and development of human embryonic tongue cells.
【作者单位】：绍兴文理学院医学院组织与胚胎学教研室;
【基金】：浙江省教育厅科研计划(Y201018535)
【分类号】：R329

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