自然衰老小鼠骨质特征及骨髓间充质干细胞生物学功能的研究

发布时间：2019-04-27 22:09

【摘要】：目的:比较观察年轻小鼠和自然衰老小鼠的骨质特征及骨髓间充质干细胞(bonemarrowmesenchymal stem cells,BMMSCs)的生物学功能。方法:分别取12月龄和2月龄C57BL/6J小鼠,Micro-CT活体扫描两组小鼠股骨毉骺端,三维图像重建后观察其骨质特征;体外分离、培养两组小鼠的BMMSCs,通过MTT、克隆形成和成骨诱导观察其增殖、自我更新和成骨分化能力;并采用实时定量基因扩增荧光检测系统(Real-time PCR)对成骨诱导14 d后的BMMSCs重要相关基因OCN进行检测。结果:与2月龄小鼠相比,12月龄小鼠出现了骨衰老症状,主要表现为骨密度及骨小梁强度下降;BMMSCs的增殖、自我更新和成骨分化能力以及OCN mRNA表达水平均明显降低。结论:BMMSCs可能在骨衰老中发挥了重要作用。
[Abstract]:Aim: to observe the characteristics of bone and the biological function of bone marrow mesenchymal stem cells (bonemarrowmesenchymal stem cells,BMMSCs) in young mice and natural aging mice. Methods: 12-month-old and 2-month-old C57BL/6J mice were used to scan the epiphysis of femur with Micro-CT in vivo. The bone characteristics were observed after three-dimensional image reconstruction. The proliferation, self-renewal and osteogenic differentiation of BMMSCs, in two groups of mice were observed by MTT, clone formation and osteogenesis induction in vitro. Real-time quantitative gene amplification fluorescence detection system (Real-time PCR) was used to detect the important BMMSCs-related gene OCN 14 days after osteogenic induction. Results: compared with the 2-month-old mice, 12-month-old mice had bone senescence symptoms, the main manifestations were decreased bone mineral density and trabecular strength, and the proliferation, self-renewal, osteogenic differentiation and expression of OCN mRNA of BMMSCs were significantly lower than those of 2-month-old mice. Conclusion: BMMSCs may play an important role in bone senescence.
【作者单位】：第四军医大学口腔医学院;遵义医学院;
【基金】：国家重点基础研究发展计划项目(973项目)(2011CB964700)
【分类号】：R363

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