NIRF可抑制乙型肝炎病毒在水动力法转染HBV小鼠模型中的复制及表达
发布时间：2019-06-08 15:12
【摘要】：目前对NIRF(Np95/ICBP-90 like RING finger protein)的研究正朝着细胞癌变进程以及表观遗传学的方向发展,但在体内NIRF对乙型肝炎病毒(HBV)的复制及表达的影响,目前尚不明确.通过高压水动力法转染HBV小鼠模型,不同时间点收集血液和肝组织标本,荧光定量PCR检测血清及肝组织中病毒载量,WesternBlot检测肝组织HBc(hepatitis B virus core protein)表达,ELISA检测血清HBeAg表达,并通过免疫组化染色检测HBsAg、HBcAg在肝组织中的定位及表达.小鼠转染pAAV-HBV1.3和NIRF以后,血清及肝组织病毒载量降低(n=3,P0.05),HBc蛋白及HBV相关抗原的表达受到抑制,说明在水动力法转染HBV小鼠模型中NIRF对HBV的复制及表达起到抑制作用,期待能为后续的HBV致病机理及治疗药物的研究与开发提供支持与帮助.
[Abstract]:At present, the study of NIRF (Np95/ICBP-90 like RING finger protein) is developing towards the process of cell carcinogenesis and epigenetics, but the effect of NIRF on the replication and expression of hepatitis B virus (HBV) in vivo is not clear at present. The blood and liver tissue samples were collected at different time points. The viral load in serum and liver tissue was detected by fluorescence quantitative PCR, the expression of HBc (hepatitis B virus core protein) in liver tissue was detected by WesternBlot, and the expression of HBeAg in serum was detected by ELISA. The localization and expression of HBsAg,HBcAg in liver tissue were detected by immunohistochemical staining. After pAAV-HBV1.3 and NIRF were transferred into mice, the viral load in serum and liver tissue decreased (n 鈮，
本文编号：2495386
[Abstract]:At present, the study of NIRF (Np95/ICBP-90 like RING finger protein) is developing towards the process of cell carcinogenesis and epigenetics, but the effect of NIRF on the replication and expression of hepatitis B virus (HBV) in vivo is not clear at present. The blood and liver tissue samples were collected at different time points. The viral load in serum and liver tissue was detected by fluorescence quantitative PCR, the expression of HBc (hepatitis B virus core protein) in liver tissue was detected by WesternBlot, and the expression of HBeAg in serum was detected by ELISA. The localization and expression of HBsAg,HBcAg in liver tissue were detected by immunohistochemical staining. After pAAV-HBV1.3 and NIRF were transferred into mice, the viral load in serum and liver tissue decreased (n 鈮，
本文编号：2495386
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