钉螺血淋巴细胞提取及其形态观察

发布时间：2019-06-25 13:20

【摘要】：利用软体组织破碎过滤法提取湖北钉螺(Oncomelania hupensis)血淋巴细胞,加入柠檬酸葡萄糖溶液作为抗凝剂,经吉氏染色和美兰染色后,用Motic B5计算机成像系统观察其形态。提取单只钉螺的血淋巴细胞,统计细胞数。结果共观察54个血淋巴细胞,可见3种形态:圆形有颗粒大细胞,直径(21.59～31.97)μm,细胞核呈卵圆形,有颗粒,核质比0.38±0.08,约占细胞总数35.95%(19/54);圆形无颗粒小细胞,直径(13.24～20.77)μm,细胞核呈卵圆形,无颗粒,核质比0.38±0.90,约占细胞总数51.63%(28/54);梭形细胞,大小(17.60～25.47)μm×(27.19～30.25)μm,细胞核呈长椭圆形,无颗粒,核质比0.44±0.20,约占细胞总数12.42%(7/54)。用过滤法提取单只钉螺血淋巴细胞均数约为19.46万个。该法是提取钉螺血淋巴细胞的一种有效方法。
[Abstract]:The blood lymphocytes of (Oncomelania hupensis) of Oncomelania hupensis were extracted by soft tissue fragmentation and filtration. The blood lymphocytes of Oncomelania hupensis were treated with citric acid and glucose solution as anticoagulant. After Ji's staining and Meilan staining, the morphology of the lymphocytes was observed by Motic B5 computer imaging system. The blood lymphocytes of a single Oncomelania hupensis were extracted and the number of cells was counted. Results A total of 54 blood lymphocytes were observed. Three kinds of morphology were observed: round granule large cells, diameter (21.59 鈮，

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