RANKL和M-CSF在CIA大鼠关节滑膜的表达及药物的干预作用
发布时间：2019-06-25 16:34
【摘要】：目的初步探讨RANKL和M-CSF在CIA大鼠关节滑膜的表达及T-614、MTX的干预作用。方法通过鸡Ⅱ型胶原和完全弗氏佐剂混合的胶原乳剂免疫大鼠,建立CIA大鼠模型,将CIA大鼠随机分为模型对照组、T-614组、MTX组和T-614+MTX组。药物干预组大鼠分别用T-614、MTX及T-614联合MTX干预3周。关节炎指数(arthritis index,AI)评分评价关节炎程度,HE染色观察踝关节组织形态学变化,X线片观察骨侵蚀情况,高频超声观察踝关节并测量滑膜厚度,Western blot法检测滑膜RANKL和M-CSF表达。结果与正常对照组相比,模型对照组AI评分、病理评分、X线评分、超声下滑膜厚度、关节滑膜RANKL,M-CSF表达均增加(P0.05);与模型对照组相比,T-614组、MTX组、T-614联合MTX组上述观察指标均降低(P0.05);与T-614组、MTX组相比,T-614联合MTX组上述观察指标均降低(P0.05);T-614组和MTX组之间上述观察指标差异无统计学意义(P0.05)。结论 RANKL和M-CSF参与了CIA大鼠关节滑膜病变的发生、发展,T-614、MTX可能通过减少细胞因子RANKL,M-CSF的生成来延缓关节滑膜病变的进展,T-614与MTX具有一定的协同效应。
[Abstract]:Objective To study the expression of RANKL and M-CSF in the joint synovium of CIA rats and the intervention of T-614 and MTX. Methods The CIA rats were randomly divided into the model control group, the T-614 group, the MTX group and the T-614 + MTX group by using the collagen emulsion mixed with the chicken type II collagen and the complete Freund's adjuvant to immunize the rat. The rats of the drug intervention group were treated with T-614, MTX and T-614 in combination with MTX for 3 weeks. Arthritis index (AI) score was used to evaluate the degree of arthritis, and the morphological changes of the ankle tissue were observed with HE staining. The bone erosion was observed by X-ray. The thickness of the synovium was measured by high-frequency ultrasound, and the expression of RANKL and M-CSF was detected by Western blot. Results Compared with the normal control group, the AI score, the pathological score, the X-ray score, the ultrasonic glide film thickness, the joint synovial RANKL and the M-CSF expression in the model control group were all increased (P0.05), and the above-mentioned observation indexes of the T-614 group, the MTX group and the T-614 combined MTX group were all decreased (P0.05). Compared with the T-614 group and the MTX group, the above-mentioned observation index of the T-614 combined MTX group was decreased (P0.05), and the difference of the observation indexes between the T-614 group and the MTX group was not statistically significant (P0.05). Conclusion RANKL and M-CSF are involved in the development, development, T-614 and MTX of the joint synovium of CIA rats.
【作者单位】： 东南大学医学院;东南大学附属中大医院风湿免疫科;
【基金】：江苏省自然基金(BK2009276)~~
【分类号】：R392.3
[Abstract]:Objective To study the expression of RANKL and M-CSF in the joint synovium of CIA rats and the intervention of T-614 and MTX. Methods The CIA rats were randomly divided into the model control group, the T-614 group, the MTX group and the T-614 + MTX group by using the collagen emulsion mixed with the chicken type II collagen and the complete Freund's adjuvant to immunize the rat. The rats of the drug intervention group were treated with T-614, MTX and T-614 in combination with MTX for 3 weeks. Arthritis index (AI) score was used to evaluate the degree of arthritis, and the morphological changes of the ankle tissue were observed with HE staining. The bone erosion was observed by X-ray. The thickness of the synovium was measured by high-frequency ultrasound, and the expression of RANKL and M-CSF was detected by Western blot. Results Compared with the normal control group, the AI score, the pathological score, the X-ray score, the ultrasonic glide film thickness, the joint synovial RANKL and the M-CSF expression in the model control group were all increased (P0.05), and the above-mentioned observation indexes of the T-614 group, the MTX group and the T-614 combined MTX group were all decreased (P0.05). Compared with the T-614 group and the MTX group, the above-mentioned observation index of the T-614 combined MTX group was decreased (P0.05), and the difference of the observation indexes between the T-614 group and the MTX group was not statistically significant (P0.05). Conclusion RANKL and M-CSF are involved in the development, development, T-614 and MTX of the joint synovium of CIA rats.
【作者单位】： 东南大学医学院;东南大学附属中大医院风湿免疫科;
【基金】：江苏省自然基金(BK2009276)~~
【分类号】：R392.3
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