口疡康对阴虚型口腔溃疡模型大鼠的作用及机制研究
发布时间：2019-06-25 19:42
【摘要】：背景:口腔溃疡是口腔科中最常见、且频繁发作的疾病。因为其病因病机尚未完全明确,当今医学对其尚无确切的治疗方法及特效药物。中医主张辩证论治和治病求本,因此中医药治疗口腔溃疡具有一定的优势。中药复方口疡康临床应用治疗阴虚火旺所导致的口腔炎症疗效显著。目的:通过建立阴虚型口腔溃疡大鼠模型,来评价口疡康的治疗作用,并从p38MAPK和ERK1/2信号通路方面探讨其作用机制,为口疡康的研究开发提供实验依据。方法:1、将20只SD大鼠随机分为2组,每组10只。模型组大鼠灌胃甲状腺片混悬液,给药21日;空白组大鼠灌胃0.9%的氯化钠溶液。第22日,模型组大鼠麻醉后,用镊子夹取直径约5mm的滤纸片在56%氢氧化钠溶液中浸泡过后,置于大鼠一侧颊黏膜处烧灼20 s;空白组大鼠不作处理。24h后观察溃疡形成情况,检测大鼠血清中cAMP、cGMP水平及红细胞膜Na+-K+-ATP酶活性。2、将72只SD大鼠随机分为6组,分别为空白组、模型组、知柏地黄丸组及口疡康高、中、低剂量组,每组12只。建立阴虚型口腔溃疡大鼠模型,评价口疡康的治疗作用,检测大鼠血清中cAMP、cGMP水平及红细胞膜Na+-K+-ATP酶的活力值,溃疡黏膜组织中TNF-α、IL-6、IL-10、EGF、TGF-β1水平及p38MAPK、ERK1/2蛋白磷酸化水平。结果:1、与空白组比较,模型组大鼠口腔黏膜形成直径5mm左右的溃疡,且出现体重逐渐减轻,饮水量及体温逐渐增加,舌偏红,烦躁多动等类似阴虚症状;血清cAMP水平明显升高,cGMP水平明显降低,红细胞膜Na+-K+-ATP酶活性明显升高(P0.01);显微镜下观察模型组大鼠口腔黏膜上皮层细胞溶解坏死并脱落,黏膜固有层下有许多炎症细胞浸润,以淋巴细胞和中性粒细胞为主,并可见血管内皮细胞坏死,纤维素性渗出,形成大量坏死组织。2、与空白组比较,模型组大鼠口腔黏膜形成溃疡,且出现类似阴虚的症状;cAMP、TNF-α、IL-6水平、Na+-K+-ATP酶的活力及p38MAPK、ERK1/2蛋白磷酸化水平明显升高,cGMP、IL-10、EGF、TGF-β1水平明显降低(P0.05,P0.01)。与模型组比较,口疡康各剂量组和知柏地黄丸组大鼠阴虚症状明显改善,溃疡愈合时间明显缩短;cAMP、TNF-α、IL-6水平、Na+-K+-ATP酶的活力及p38MAPK、ERK1/2蛋白磷酸化水平明显降低,cGMP、IL-10、EGF、TGF-β1水平明显升高(P0.05,P0.01)。结论:1、本研究成功建立了阴虚型口腔溃疡大鼠模型,该模型实施简便可行、容易重复。2、口疡康对阴虚型口腔溃疡模型大鼠有明显的治疗作用,其机制可能是通过调节机体代谢、相关细胞因子水平及p38MAPK和ERK1/2蛋白磷酸化水平实现的。
[Abstract]:Background: oral ulcer is the most common and frequent disease in stomatology. Because its etiology and pathogenesis are not completely clear, there is no exact treatment and special drugs in today's medicine. Traditional Chinese medicine advocates dialectical treatment and treatment of diseases, so traditional Chinese medicine has certain advantages in the treatment of oral ulcer. The clinical application of traditional Chinese medicine compound Kou Yang Kang is effective in the treatment of oral inflammation caused by deficiency of yin and fire. Objective: to evaluate the therapeutic effect of Kou Yangkang by establishing a rat model of oral ulcer with yin deficiency type, and to explore its mechanism from the aspects of p38MAPK and ERK1/2 signal pathway, so as to provide experimental basis for the research and development of Kou Yangkang. Methods: 1. 20 SD rats were randomly divided into two groups with 10 rats in each group. The rats in the model group were given the suspension of thyroid tablets for 21 days, and the rats in the blank group were given 0.9% sodium chloride solution. On the 22nd day, after anaesthesia, the filter paper about 5mm was clipped with tweezers and soaked in 56% sodium hydroxide solution, and then burned in the buccal mucosa of rats for 20 s. The rats in the blank group were not treated. 24 hours later, the formation of ulcer was observed, and the level of cAMP,cGMP in serum and the activity of Na-K-ATP enzyme in erythrocyte membrane were measured. 2. 72 SD rats were randomly divided into 6 groups: blank group, model group, Zhibai Dihuang pill group and Kou Yang Kang Gao, medium and low dose groups with 12 rats in each group. The rat model of oral ulcer with yin deficiency was established to evaluate the therapeutic effect of Kou Yangkang. The levels of cAMP,cGMP in serum, the activity of Na-K-ATP enzyme in erythrocyte membrane, the levels of TNF- 伪, IL-6,IL-10,EGF,TGF- 尾 1 in ulcer mucosa and the phosphorylation of p38MapK and ERK1 鈮，
本文编号：2505952
[Abstract]:Background: oral ulcer is the most common and frequent disease in stomatology. Because its etiology and pathogenesis are not completely clear, there is no exact treatment and special drugs in today's medicine. Traditional Chinese medicine advocates dialectical treatment and treatment of diseases, so traditional Chinese medicine has certain advantages in the treatment of oral ulcer. The clinical application of traditional Chinese medicine compound Kou Yang Kang is effective in the treatment of oral inflammation caused by deficiency of yin and fire. Objective: to evaluate the therapeutic effect of Kou Yangkang by establishing a rat model of oral ulcer with yin deficiency type, and to explore its mechanism from the aspects of p38MAPK and ERK1/2 signal pathway, so as to provide experimental basis for the research and development of Kou Yangkang. Methods: 1. 20 SD rats were randomly divided into two groups with 10 rats in each group. The rats in the model group were given the suspension of thyroid tablets for 21 days, and the rats in the blank group were given 0.9% sodium chloride solution. On the 22nd day, after anaesthesia, the filter paper about 5mm was clipped with tweezers and soaked in 56% sodium hydroxide solution, and then burned in the buccal mucosa of rats for 20 s. The rats in the blank group were not treated. 24 hours later, the formation of ulcer was observed, and the level of cAMP,cGMP in serum and the activity of Na-K-ATP enzyme in erythrocyte membrane were measured. 2. 72 SD rats were randomly divided into 6 groups: blank group, model group, Zhibai Dihuang pill group and Kou Yang Kang Gao, medium and low dose groups with 12 rats in each group. The rat model of oral ulcer with yin deficiency was established to evaluate the therapeutic effect of Kou Yangkang. The levels of cAMP,cGMP in serum, the activity of Na-K-ATP enzyme in erythrocyte membrane, the levels of TNF- 伪, IL-6,IL-10,EGF,TGF- 尾 1 in ulcer mucosa and the phosphorylation of p38MapK and ERK1 鈮，
本文编号：2505952
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