重组人促红细胞生成素对高氧肺损伤新生大鼠细胞凋亡及蛋白激酶B表达的影响

发布时间：2019-06-26 22:25

【摘要】：目的支气管肺发育不良(bronchopulmonary dysplasia,BPD)是新生儿呼吸系统常见疾病,文中探讨rhEPO对高氧肺损伤新生大鼠肺细胞凋亡和蛋白激酶B(又称Akt)表达的影响,为BPD的防治提供实验依据。方法将新生大鼠给予60%高氧暴露,rhEPO组皮下注射rhEPO,采用TUNEL法检测肺细胞凋亡和RT-PCR法检测Akt mRNA表达水平。结果①高氧组新生大鼠肺细胞凋亡指数为44.312±12.337,明显高于空气组的12.810±4.353(P0.01);rhEPO组凋亡指数为23.371±9.508,明显低于高氧组(P0.01)。②高氧组肺组织Akt mRNA表达水平为0.462±0.122,明显低于空气组的1.236±0.357(P0.01);rhEPO组肺组织Akt mRNA表达水平为0.893±0.235,明显高于高氧组(P0.01)。结论高氧可引起新生大鼠肺细胞凋亡增多,rhEPO可明显减少肺细胞凋亡,其机制可能与肺组织Akt mRNA表达有关。
[Abstract]:Objective Bronchopulmonary dystrophy (bronchopulmonary dysplasia,BPD) is a common disease of neonatal respiratory system. The effects of rhEPO on apoptosis and protein kinase B (Akt) expression in neonatal rats with hyperoxia lung injury were investigated in order to provide experimental basis for the prevention and treatment of BPD. Methods Neonatal rats were exposed to 60% hyperoxia. The apoptosis of lung cells was detected by TUNEL and the expression of Akt mRNA was detected by RT-PCR in rhEPO group by subcutaneous injection of rhEPO,. Results 1the apoptosis index of neonatal rats in hyperoxia group was 44.312 卤12.337, which was significantly higher than that in air group (12.810 卤4.353) (P 0.01), which was significantly lower than that in hyperoxia group (23.371 卤9.508), which was significantly lower than that in hyperoxia group (P 0.01). 2 the expression level of Akt mRNA in hyperoxia group was 0.462 卤0.122, which was significantly lower than that in air group (1.236 卤0.357). The expression level of Akt mRNA in lung tissue of rhEPO group was 0.893 卤0.235, which was significantly higher than that of hyperoxia group (P 0.01). Conclusion Hyperoxia can increase the apoptosis of lung cells in neonatal rats, and rhEPO can significantly reduce the apoptosis of lung cells, which may be related to the expression of Akt mRNA in lung tissue.
【作者单位】：桂林医学院附属医院新生儿科;
【基金】：广西壮族自治区卫生厅重点课题(2011011)
【分类号】：R363

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