内蒙古2009年流感病原监测及甲型H1N1流感血凝素基因分析

发布时间：2019-08-17 16:10

【摘要】：目的探讨2009年流感流行病毒型别及亚型在内蒙古流感样患者中的流行情况及病毒型内血凝素(HA)基因变异情况。方法采集流感样病例咽拭子标本,用Real-timePCR进行样本的检验,阳性标本进行鸡胚病毒分离培养,选取代表性毒株做病毒血凝素(HA)基因测序分析。结果 Real-time PCR方法共检测到阳性标本419份,其中甲型H1N1流感182份,占阳性样本总数的43.44%。对419份阳性样本全部进行鸡胚分离培养,获得阳性毒株162株,阳性分离率为38.66%。经鉴定甲型H1N1 155株,占阳性毒株的95.68%;甲型H1N1与季节性H3N2混合2株,季节性H3N24株,B型毒株1株。对其中3株甲型H1N1毒株进行HA测序后分析发现病毒分离株与参比毒株的HA基因序列具有高度同源性。结论 2009年在内蒙古地区流行的主要是甲型H1N1病毒,兼有其他亚型出现。甲型H1N1流感与当年的流行株无大的变异发生。
[Abstract]:Objective to investigate the prevalence of influenza epidemic virus types and subtypes in Inner Mongolia influenza-like patients in 2009 and the variation of hemagglutinin (HA) gene in viral type. Methods the throat swabs of influenza cases were collected and tested by Real-timePCR. The positive samples were isolated and cultured for chicken embryo virus, and the representative strains were selected for virus hemagglutinin (HA) gene sequencing. Results A total of 419 positive samples were detected by Real-time PCR, of which 182 were H1N1 A influenza, accounting for 43.44% of the total positive samples. All 419 positive samples were isolated and cultured in chicken embryos, and 162 positive strains were obtained, the positive isolation rate was 38.66%. 155 strains of type A H1N1 were identified, accounting for 95.68% of the positive strains, and 2 strains of type A H1N1 and 2 strains of seasonal H3N2, 1 strain of seasonal H3N24 and 1 strain of type B were identified. After HA sequencing of three A H1N1 strains, it was found that the HA gene sequences of the virus isolates were highly homologous to those of the reference strains. Conclusion in 2009, the main epidemic in Inner Mongolia was H1N1 A virus, and other subtypes appeared. There was no significant variation between influenza A (H1N1) and the epidemic strain of that year.
【作者单位】：内蒙古自治区疾病预防控制中心;
【分类号】：R373.13

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