上调miRNA-21对骨髓间充质干细胞的影响
发布时间：2021-07-01 18:06
目的探讨miRNA-21对大鼠骨髓间充质干细胞（bone marrow mesenchymal stem cells,BMSCs）特性的影响。方法 BMSCs使用DMEM液进行培养传代,流式细胞仪检测BMSCs的表面相关抗原表达。建立miRNA-21慢病毒干扰载体（LV-miRNA-21）,并转染BMSCs。分3组细胞进行对比实验[CK（正常组）、NC（对照组）、miRNA-21组],分别培养3 d、7 d和14 d;利用免疫荧光染色技术和Western-blotting技术检测并比较肌钙蛋白在3组细胞中3个时间点的表达情况,噻唑蓝（MTT）法检测3组BMSCs的增殖情况,运用流式细胞技术检测3组BMSCs的细胞周期及凋亡情况。结果 BMSCs呈长梭形不规则形态,表现出干细胞特异性抗原CD90和CD29阳性,CD45阴性。成功构建LV-miRNA-21;发现在miRNA-21组BMSCs中经诱导分化后肌钙蛋白表达量明显高于CK组和NC组（P<0.05）,在miRNA-21组中表现出细胞的增殖加快,但凋亡速度比CK组和NC组减慢。结论 miRNA-21影响BMSCs的增殖和凋亡,可...
【文章来源】：中国胸心血管外科临床杂志. 2020,27(08)CSCD
【文章页数】：8 页
【参考文献】：
期刊论文
[1]Molecular mechanisms of mesenchymal stem cell differentiation towards osteoblasts[J]. Maya Fakhry,René Buchet,David Magne,Eva Hamade,Bassam Badran. World Journal of Stem Cells. 2013(04)
[2]Differentiation of bone marrow mesenchymal stem cells induced by myo-cardial medium under hypoxic conditions[J]. Xiao-jie XIE~1 Jian-an WANG~(1,3)Jiang CAO~2 Xing ZHANG~2 1 Division of Cardiology,the 2nd Affiliated Hospital,College of Medicine,Zhejiang University Hangzhou 310009,China;2 Clinical Research Institute,Sir Run Run Shaw Hospital,College of Medicine,Zhejiang University,Hangzhou 310016,China. Acta Pharmacologica Sinica. 2006(09)
本文编号：3259615
【文章来源】：中国胸心血管外科临床杂志. 2020,27(08)CSCD
【文章页数】：8 页
【参考文献】：
期刊论文
[1]Molecular mechanisms of mesenchymal stem cell differentiation towards osteoblasts[J]. Maya Fakhry,René Buchet,David Magne,Eva Hamade,Bassam Badran. World Journal of Stem Cells. 2013(04)
[2]Differentiation of bone marrow mesenchymal stem cells induced by myo-cardial medium under hypoxic conditions[J]. Xiao-jie XIE~1 Jian-an WANG~(1,3)Jiang CAO~2 Xing ZHANG~2 1 Division of Cardiology,the 2nd Affiliated Hospital,College of Medicine,Zhejiang University Hangzhou 310009,China;2 Clinical Research Institute,Sir Run Run Shaw Hospital,College of Medicine,Zhejiang University,Hangzhou 310016,China. Acta Pharmacologica Sinica. 2006(09)
本文编号：3259615
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