Inter-chain disulfide bond improved protein trans-splicing i

发布时间：2021-07-21 09:11

　　Protein trans-splicing based dual-vector factor VIII（FVIII） gene delivery is adversely affected by less efficiency of protein splicing.We sought to increase the amount of spliced FVIII protein and plasma coagulation activity in dual-vector FVIII transgene in mice by means of strengthening the interaction of inteins,protein splicing elements,thereby facilitating protein trans-splicing.Dual-vector delivery of the FVIII gene in cultured cells showed that replacement of Met226 in the heavy chain and... 

【文章来源】：Science China（Life Sciences）. 2013,56(03)SCI

【文章页数】：6 页

【文章目录】：
1 Materials and methods
    1.1 Materials
    1.2 Vector construction
    1.3 Animal procedures
    1.4 Plasma FVIII antigen assay
    1.5 Plasma FVIII coagulation activity assay
    1.6 Statistical analysis
2 Results
    2.1 Structures of BDD-FVIII and its intein fused heavy and light chains
    2.2 Transgenes produced plasma concentrations of BDD-FVIII light and heavy chains
    2.3 Dual-vector transgene generated plasma coagula-tion activity
3 Discussion



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