NGF在大鼠慢性偏头痛模型中的表达及机制研究
发布时间：2021-08-20 17:49
背景:我们前期研究发现,三叉神经脊束尾核（trigeminal nucleus caudalis,TNC）中酸敏感离子通道3（acid-sensing ion channel 3,ASIC3）参与了反复发作性偏头痛的病理过程。神经生长因子（nerve growth factor,NGF）可以诱导许多疼痛疾病的痛觉敏化,并且可以调控ASIC3的表达。然而,NGF在慢性偏头痛（chronic migraine,CM）中的作用尚不清楚。所以我们探究了CM大鼠TNC部位NGF的表达,NGF对ASIC3的调控作用,及相关的信号通路在炎性汤（inflammatory soup,IS）诱导的皮肤痛觉过敏中的作用。方法:1.通过硬脑膜反复滴注7日IS建立CM大鼠模型,模拟CM的发作。von Frey测定大鼠眶周及足底的痛阈值,以此评价模型的建立。2.利用PCR,Western blot检测TNC部位NGF m RNA水平及蛋白水平,免疫荧光检测NGF在TNC神经元中的定位情况。3.CM大鼠侧脑室注射NGF中和抗体（anti-NGF neutralizing antibody,anti-NGF）,von...
【文章来源】：重庆医科大学重庆市
【文章页数】：51 页
【学位级别】：硕士
【部分图文】：
Sham组和CM组的痛阈值
NGF在TNC部位的表达和定位Fig.2TheexpressionandlocalizationofNGFintheTNC(A)ThemRNAofNGFintheTNCwasdetectedwithq-PCR.Asshownbythestatisticalanalysis,theNGFmRNAexpressionlevelintheTNCwassignificantlyhigherintheCMgroupthanintheShamgroup.(B)TheNGFproteinexpressionlevelintheTNCwassignificantlyhigherintheCMgroupthanintheShamgroup.(n=6pergroup,*p<0.05,**p<0.01vs
图 3.Anti-NGF 对痛阈值的影响Fig. 3 Effects of anti-NGF treatment on the pain thresholds(A) The periorbital pain threshold was significantly lower in the CM and CM+PBS groupan in the Sham group, and there was no significant difference between the CM and CM+Pgroups. The pain threshold of the periorbital region was significantly higher in theM+anti-NGF MD (0.5 g) and CM+anti-NGF HD (1 g) groups than in the CM+PBS grohere were no significant differences between the CM+anti-NGF MD and CM+anti-NGF Hgroups. The pain threshold of the periorbital region did not differ significantly between thCM+anti-NGF LD (0.1 g) group and the CM+PBS group. (B) The hind paw withdrawareshold was significantly lower in the CM and CM+PBS groups than in the Sham group, there was no significant difference between the CM and CM+PBS groups. The withdrawthreshold of the hind paw was significantly higher in the CM+anti-NGF MD (0.5 g) andCM+anti-NGF HD (1 g) groups than in the CM+PBS group. There were no significantifferences between the CM+anti-NGF MD and CM+anti-NGF HD groups. The pain threshf the hind paw did not differ significantly between the CM+anti-NGF LD (0.1 g) group athe CM+PBS group. (n=6 per group, ** p < 0.01 vs Sham group; ## p < 0.01 vs CM+PBS
本文编号：3353951
【文章来源】：重庆医科大学重庆市
【文章页数】：51 页
【学位级别】：硕士
【部分图文】：
Sham组和CM组的痛阈值
NGF在TNC部位的表达和定位Fig.2TheexpressionandlocalizationofNGFintheTNC(A)ThemRNAofNGFintheTNCwasdetectedwithq-PCR.Asshownbythestatisticalanalysis,theNGFmRNAexpressionlevelintheTNCwassignificantlyhigherintheCMgroupthanintheShamgroup.(B)TheNGFproteinexpressionlevelintheTNCwassignificantlyhigherintheCMgroupthanintheShamgroup.(n=6pergroup,*p<0.05,**p<0.01vs
图 3.Anti-NGF 对痛阈值的影响Fig. 3 Effects of anti-NGF treatment on the pain thresholds(A) The periorbital pain threshold was significantly lower in the CM and CM+PBS groupan in the Sham group, and there was no significant difference between the CM and CM+Pgroups. The pain threshold of the periorbital region was significantly higher in theM+anti-NGF MD (0.5 g) and CM+anti-NGF HD (1 g) groups than in the CM+PBS grohere were no significant differences between the CM+anti-NGF MD and CM+anti-NGF Hgroups. The pain threshold of the periorbital region did not differ significantly between thCM+anti-NGF LD (0.1 g) group and the CM+PBS group. (B) The hind paw withdrawareshold was significantly lower in the CM and CM+PBS groups than in the Sham group, there was no significant difference between the CM and CM+PBS groups. The withdrawthreshold of the hind paw was significantly higher in the CM+anti-NGF MD (0.5 g) andCM+anti-NGF HD (1 g) groups than in the CM+PBS group. There were no significantifferences between the CM+anti-NGF MD and CM+anti-NGF HD groups. The pain threshf the hind paw did not differ significantly between the CM+anti-NGF LD (0.1 g) group athe CM+PBS group. (n=6 per group, ** p < 0.01 vs Sham group; ## p < 0.01 vs CM+PBS
本文编号：3353951
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