大麻素受体2对重症急性胰腺炎的影响

发布时间：2018-01-09 12:21

本文关键词：大麻素受体2对重症急性胰腺炎的影响　出处：《兰州大学》2014年硕士论文　论文类型：学位论文

【摘要】：目的：主要探讨大麻素受体2(Cannabinoid receptor2, CB2)对重症急性胰腺炎(Severe acute pancreatitis, SAP)导致的肝、肾、肺损伤的作用。方法：雄性SPF级SD大鼠96只按随机数字表法分成4组,对照组：仅开腹后翻动胰腺组织后关腹；SAP组：4%牛磺胆酸钠逆行注入胰管建立SAP模型；HU-308组：腹腔注射HU-3082.5mg/kg,1h后建立SAP模型；AM-630组：腹腔注射AM-6302.5mg/kg,1h后建立SAP模型。分别在3h、6h、12h分离血清低温保存,检测血清中淀粉酶(Amylase, AMY),肿瘤坏死因子-α (Tumor necrosis factor-α, TNF-α)、白介素-6(Interleukin-6, IL-6)、谷丙转氨酶(Alanine aminotransferase, ALT)、谷草转氨酶(Aspartate Transaminase, AST)、血尿素氮(Blood urea nitrogen, BUN)、肌酐(Creatinine, Cr)水平。取新鲜左肺下叶匀浆后检测髓过氧化物酶(Myeloperoxidase, MPO)水平,将右肺下叶制作成HE病理切片并进行病理学评分。其余肺组织称重后进行干燥处理,测量湿干重之比。结果：在每个时间点,SAP组、AM-630组、HU-308组中AMY、TNF-α、 IL-6、MPO、肺组织湿干重比值和病理学评分均高于对照组(P0.05);在3h时间点,SAP组、AM-630组、HU-308组中ALT、AST、BUN、Cr水平分别与对照组相比较,无明显差异(P0.05),在6h、12h时间点SAP组、AM-630组、HU-308组中ALT、AST、BUN、Cr水平均高于对照组(P0.05)。在3h时间点,HU-308组中AMY、TNF-α、IL-6、MPO、肺组织湿干重比值、病理学评分均低于SAP组(P0.05),ALT、AST、BUN、Cr水平与SAP组无明显差异(P0.05)；在6h、12h时间点,HU-308组中AMY、TNF-α、IL-6、MPO、肺组织湿干重比值、病理学评分、ALT、AST、BUN和Cr水平均低于SAP组(P0.05)。在3h时间点,AM-630组中AMY、TNF-α、IL-6和MPO水平高于SAP组(P0.05),肺组织湿干重比值、病理学评分、ALT、AST、BUN和Cr水平与SAP组相比较,无统计学差异(P0.05)；在6h时间点,AM-630组中AMY、TNF-α、IL-6、MPO、肺组织湿干重比值、病理学评分、AST、BUN和Cr水平高于SAP组(P0.05), ALT水平与SAP组相比较无统计学差异(P0.05)；在12h时间点,AM-630组中所检测的所有指标均高于SAP组(P0.05)。结论：CB2受体的激活对SAP导致的肝、肾、肺损伤起到保护作用,CB2受体被抑制后肝、肾、肺损伤的程度加重。
[Abstract]:Objective: to investigate cannabinoid receptor2 of cannabinoid receptor 2. The effect of CB2 on liver, kidney and lung injury induced by severe acute pancreatitis (SAP). Methods: 96 male SPF SD rats were randomly divided into 4 groups. SAP model was established by retrograde injection of sodium taurocholate into pancreatic duct in SAP group. In HU-308 group, SAP model was established after intraperitoneal injection of HU-3082.5 mg / kg for 1 hour. In AM-630 group, the SAP model was established after intraperitoneal injection of AM-6302.5mg / kg for 1 h. Serum amylase Amylase, amylase, tumor necrosis factor- 伪 Tumor necrosis factor- 伪 (TNF- 伪) were detected. Interleukin-6, IL-6, alanine aminotransferase (alt). Aspartate transaminase (AST), blood urea nitrogenin (bunn). The levels of myeloperoxidase (MPO) were measured after fresh left lower lung homogenate. The lower lobe of the right lung was made into HE pathological sections and the other lung tissues were dried after weighing and the ratio of wet and dry weight was measured. Results: AMYTNF- 伪 and IL-6 MPO were detected in AM-630 group and HU-308 group at each time point. The wet / dry weight ratio and pathological score of lung tissue were higher than that of control group (P 0.05). At 3 h, there was no significant difference in the levels of alt ASTT bun Cr between the SAP group and the control group (P 0. 05) in AM-630 group and HU-308 group (P < 0. 05, P < 0. 05, P 0. 05, P 0. 05, P < 0. 05). At the time point of 6 h and 12 h, the levels of alt ASTT bun Cr in the SAP group were higher than those in the control group (P 0.05), and at the 3h time point, the levels of alt ASTT bun Cr in the HU-308 group were higher than those in the control group (P < 0.05). In HU-308 group, AMYT TNF- 伪 IL-6 MPO, lung wet / dry weight ratio and pathological score were lower than those in SAP group (P 0.05). There was no significant difference between Cr level and SAP group (P 0.05). In HU-308 group, AMYT TNF- 伪 IL-6 MPO, lung wet / dry weight ratio, pathological score and AST were measured at 6 h and 12 h. The levels of BUN and Cr in AM-630 group were lower than those in SAP group (P 0.05). The levels of IL-6 and MPO were higher than that of SAP group (P 0.05), the ratio of wet and dry weight of lung tissue, and the pathological scores of alt ASTT bun and Cr were compared with those of SAP group. There was no statistical difference (P 0.05). AM-630 group was treated with AMYT TNF- 伪 IL-6 MPO, lung wet / dry weight ratio, pathological score and AST. The levels of BUN and Cr in SAP group were higher than those in SAP group (P 0.05), and there was no significant difference in ALT level between SAP group and ALT group (P 0.05). All the indexes detected in AM-630 group at 12h were higher than those in SAP group (P 0.05). Conclusion the activation of the CB2 receptor can protect the liver, kidney and lung injury induced by SAP. The degree of liver, kidney and lung injury after the inhibition of CB2 receptor is aggravated.
【学位授予单位】：兰州大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R657.51

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