阿魏酸在慢性创面中的治疗作用研究

发布时间：2018-01-28 22:31

本文关键词： 阿魏酸糖尿病慢性难愈性创面　出处：《桂林医学院》2015年硕士论文　论文类型：学位论文

【摘要】：目的:研究阿魏酸对糖尿病大鼠难愈性创面及兔耳缺血性难愈性创面是否有促愈合作用及可能机制。方法:20只雄性SD大鼠设为正常对照组,80只经高糖高脂饲料喂养联合45mg/kg链脲佐菌素腹腔注射诱导的糖尿病大鼠随机平均分为4组:糖尿病对照组、凡士林涂抹组、5%阿魏酸涂抹组、20mg/kg阿魏酸灌胃组。大鼠鼠背制作2cm×2cm全层皮肤缺损创面,每日创面换药并给药干预,正常对照组及糖尿病对照组仅换药处理。40只雄性新西兰大耳白兔,随机平均分为4组:凡士林组、缺血对照组、5%阿魏酸组、贝复济(rb-bFGF)组;兔耳经缺血处理并验证缺血效果后制作兔耳腹侧创面,每日创面换药并给药干预,缺血对照组仅换药处理。两个动物实验均于术后1、5、8、11、14、17天拍照观察,记录各组创面愈合情况并取材,计算创面各时间点愈合率,比较各组创面愈合时间。取材时将标本由中线一分为二,一半固定后行苏木精-伊红染色观察创面愈合情况及免疫组织化学染色显示组织中血管内皮生长因子(Vascular endothelial growth factor.VEGF)、转化生长因子-β1(transforming growth factor-beta 1.TGFβ1)、血小板-内皮细胞粘附分子(Platelet endothelial cell adhesion molecule-1,PECAM-1/CD31)的表达情况,一半用于提取RNA后PCR检测VEGF、TGFβ1、缺氧诱导因子-1α(hypoxia inducible factor-1α.HIF-1α)的表达。结果:大鼠实验结果:大体观察、各组大鼠各时间点创面愈合率、各组大鼠创面愈合时间及造创后8天各组创面组织HE染色均表明5%阿魏酸组及20mg/kg阿魏酸灌胃组创面愈合较糖尿病对照组及凡士林组愈合速度快,慢于正常对照组,P0.05;各组创面组织免疫组织化学染色表明5%阿魏酸组及20mg/kg阿魏酸灌胃组VEGF及TGFβ1含量明显较糖尿病对照组及凡士林组高,低于正常对照组,P0.05;PCR结果可见5%阿魏酸组及20mg/kg阿魏酸灌胃组VEGF、TGFβ1、HIF-1α表达较糖尿病对照组及凡士林组高,低于正常对照组,P0.05。兔实验结果:大体观察、各组兔耳创面愈合时间及造创后11天各组创面组织HE染色均表明5%阿魏酸组愈合速度与贝复济组相近、明显较缺血对照组及凡士林组快,P0.05;造创后各组创面组织免疫组织化学染色表明5%阿魏酸组VEGF含量及CD31标记血管数与贝复济组接近,明显高于缺血对照组及凡士林组,P0.05;PCR结果可见5%阿魏酸组VEGF、TGFβ1、HIF-1α表达与贝复济组接近,明显高于缺血对照组及凡士林组,P0.05。结论:阿魏酸能通过提高VEGF、TGFβ1及HIF-1α等与新生血管密切相关的蛋白表达而促进创面血管生成进而促进慢性创面愈合。
[Abstract]:Objective: to study whether ferulic acid can promote healing of refractory wound and ischemic refractory wound of rabbit ear in diabetic rats. Methods: 20 male Sprague-Dawley rats were set as normal control group. 80 diabetic rats fed with high glucose and high fat diet combined with 45 mg / kg streptozotocin were randomly divided into 4 groups: diabetic control group and vaseline smear group with 5% ferulic acid. Rats in 20mg / kg ferulic acid group were treated with 2 cm 脳 2 cm full-thickness skin defect wounds. The wounds were changed daily and treated with drugs. 40 male New Zealand white rabbits were randomly divided into 4 groups: vaseline group and ischemic control group (5% ferulic acid). Rb-bFGFgroup; The rabbit ear was treated with ischemia and the effect of ischemia was verified. The wound surface of rabbit ear was changed daily and treated with drugs. The ischemic control group was treated only by dressing. The two animal experiments were carried out at 1 minute after operation (81114). The wound healing rate was calculated at each time point, and the wound healing time was compared. The specimen was divided into two parts from the middle line. The wound healing was observed by hematoxylin-eosin staining and the expression of vascular endothelial growth factor (VEGF) in the tissue was detected by immunohistochemical staining. Vascular endothelial growth factor.VEGF. Transforming growth factor- 尾 1 (TGF- 尾 1) was transformed into growth factor-beta (1. TGF- 尾 1). Platelet endothelial cell adhesion molecule-1. Half of the expression of PECAM-1 / CD31 was used to detect VEGF TGF- 尾 1 by PCR after RNA extraction. Expression of hypoxia inducible factor-1 伪. HIF-1 伪. Results: experimental results: gross observation. The wound healing rate at each time point in each group was higher than that in the control group. The wound healing time and wound tissue HE staining showed that the wound healing rate of 5% mg / kg ferulic acid group and 20 mg / kg ferulic acid group was faster than that of diabetic control group and vaseline group. . It was slower than the normal control group (P 0.05). Immunohistochemical staining of wound tissue showed that the contents of VEGF and TGF 尾 1 in 5% ferulic acid group and 20mg / kg ferulic acid group were significantly higher than those in diabetic control group and vaseline group. It was lower than the normal control group (P 0.05). PCR results showed that the expression of VEGF TGF- 尾 1 HIF-1 伪 in 5% ferulic acid group and 20mg / kg ferulic acid group was higher than that in diabetic control group and vaseline group, and was lower than that in normal control group. P0.05.The results of rabbit experiment showed that the healing time of rabbit ear wound in each group and HE staining of wound tissue in each group 11 days after operation showed that the healing speed of 5% ferulic acid group was similar to that of Befuji group. Compared with ischemic control group and vaseline group, P 0.05 was faster than that in ischemic control group. The immunohistochemical staining of wound tissue in each group showed that the VEGF content and the number of CD31 labeled vessels in the 5% ferulic acid group were similar to those in the Beifuji group, which were significantly higher than those in the ischemic control group and vaseline group (P 0.05). The results of PCR showed that the expression of VEGF TGF- 尾 1 HIF-1 伪 in 5% ferulic acid group was similar to that in epigastric group, and was significantly higher than that in ischemic control group and vaseline group. Conclusion: ferulic acid can promote wound angiogenesis and promote chronic wound healing by increasing the expression of VEGF TGF- 尾 1 and HIF-1 伪.
【学位授予单位】：桂林医学院
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R641

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