内毒素吸附剂SPV对创伤失血性休克模型大鼠肠黏膜损伤的保护作用

发布时间：2018-01-30 15:25

  本文关键词： 内毒素吸附剂 创伤失血性休克 内毒素 一氧化氮 二胺氧化酶 肿瘤坏死因子α 大鼠　出处：《中国药房》2016年10期 　论文类型：期刊论文

【摘要】：目的:研究内毒素吸附剂SPV对创伤失血性休克模型大鼠肠黏膜损伤的保护作用。方法:将0.5 mg多黏菌素B、5 mg维生素B6、0.3 g蒙脱石散溶于生理盐水中制成5 ml溶液,即SPV低剂量;中、高剂量药物成分为低剂量的2、3倍。取大鼠随机分为正常对照组、模型组和SPV低、中、高剂量组,每组10只。除正常对照组外,其余各组大鼠通过股动脉插管放血术复制创伤失血性休克模型,建模前30 min SPV各剂量组大鼠ig相应剂量的SPV。复苏后1、4、8、16 h,检测各组大鼠血清中一氧化氮(NO)、二胺氧化酶(DAO)、内毒素及肿瘤坏死因子α(TNF-α)水平,观察正常对照组、模型组和SPV高剂量组大鼠肠黏膜细胞特征和损伤指数。结果:与正常对照组比较,模型组大鼠肠黏膜细胞出现坏死的病理现象,血清中NO、DAO、内毒素和TNF-α水平均升高(P0.05)。与模型组比较,SPV中、高剂量组大鼠复苏后1、4、8、16 h肠黏膜细胞损伤程度明显减轻,血清中NO、DAO、内毒素和TNF-α水平均降低(P0.05);其余各组比较差异无统计学意义(P0.05)。结论:内毒素吸附剂SPV能够降低创伤失血性休克模型大鼠血清中NO、DAO、内毒素及TNF-α水平,对创伤失血性休克引起的肠黏膜损伤有保护作用。
[Abstract]:Objective: to study the protective effect of endotoxin adsorbent SPV on intestinal mucosal injury in rats with traumatic hemorrhagic shock. 0. 3 g montmorillonite powder dissolved in normal saline to make 5 ml solution, that is, low dose of SPV; Rats were randomly divided into normal control group, model group and SPV low, medium and high dose groups, 10 rats in each group, except the normal control group. The model of traumatic hemorrhagic shock was established by femoral artery catheterization and bloodletting in other groups. The rats in each dose group of 30 min SPV were given the corresponding dose of SPV before 30 min before resuscitation. The serum levels of nitric oxide (no), diamine oxidase (diamine oxidase), endotoxin (endotoxin) and tumor necrosis factor 伪 (TNF- 伪) in the serum of rats in each group were measured at 16 h, and the normal control group was observed. Results: compared with the normal control group, the intestinal mucosal cells in the model group and the high dose group of SPV were necrotic. The levels of endotoxin and TNF- 伪 were both increased (P 0.05). Compared with the model group, the injury degree of intestinal mucosal cells in the high dose group was significantly reduced after resuscitation at 1: 4 and 816 h after resuscitation. The levels of NONDAO, endotoxin and TNF- 伪 in serum were all decreased (P 0.05). Conclusion: endotoxin adsorbent SPV can reduce serum NO-DAO in rats with traumatic hemorrhagic shock. The levels of endotoxin and TNF- 伪 have protective effect on intestinal mucosal injury induced by traumatic hemorrhagic shock.
【作者单位】： 唐山市开滦总医院麻醉科;
【基金】：河北省2014年度医学科学研究重点课题计划(No.ZD20140064)
【分类号】：R-332;R605.971
【正文快照】： 重度创伤失血性休克(Traumatic and hemorrhagic shockTHS)是由于创伤或者疾病引起的有效循环血量不足,导致人体微循环障碍、体液因子水平失衡而引起的综合征。临床表现为各器官的缺血缺氧、血压下降、面色苍白、脉搏微弱等症状,严重者甚至出现神志昏迷。失血性休克是多器官功，

本文编号：1476558