大鼠液压冲击脑损伤Nrf2、HO-1和NQO-1动态表达变化及意义

发布时间：2018-02-03 14:40

本文关键词： 颅脑液压冲击伤核因子相关因子血红素氧化酶- 磷酸酰胺腺嘌呤二核苷酸醌氧化还原酶-　出处：《第三军医大学学报》2016年18期 　论文类型：期刊论文

【摘要】：目的探讨大鼠液压冲击伤后水肿脑组织核因子2相关因子2(nuclear factor erythroid-2-related factor 2,Nrf2)、血红素氧化酶-1(heme oxygenase-1,HO-1)和磷酸酰胺腺嘌呤二核苷酸醌氧化还原酶-1(NADPH quinineoxidoreductase-1,NQO-1)的表达变化及其意义。方法成年雄性SD大鼠56只制作液压冲击颅脑损伤模型,术后1 h、6 h、12 h、24 h、3 d、7 d用干湿质量法测定脑组织含水量,Western blot测定Nrf2胞浆、胞核蛋白以及HO-1、NQO-1蛋白;用实时荧光定量PCR测定Nrf2 mRNA表达。结果自冲击伤后6 h,脑组织含水量开始增加,24h达高峰,持续至3 d,然后开始下降(P0.05)。组织学观察印证了脑水肿趋势。Western blot检测结果显示胞浆Nrf2蛋白于伤后12 h开始持续降低,而胞核Nrf2蛋白则于1h开始升高,24 h达到高峰,3 d时降低,7 d后明显降低,但仍高于NC组(P0.05)。HO-1和NQO-1蛋白表达与胞核Nrf2蛋白趋势一致。实时荧光定量PCR显示TBI组术后各时间点Nrf2 mRNA表达水平无明显变化(P0.05)。结论液压冲击伤后出现核因子Nrf2转位激活,可能通过上调HO-1、NQO-1蛋白表达发挥神经保护作用。
[Abstract]:Objective to investigate the brain tissue nuclear factor-2 related factor 2 (NF-2) in rats with edema after hydraulic blast injury. Nuclear factor erythroid-2-related factor 2. Nrf2, heme oxygenase-1 of heme oxygenase-1. HO-1) and adenine dinucleotide quinone oxidoreductase -1 (NADPH quinineoxidoreductase-1). Methods 56 adult male Sprague-Dawley rats were used to make the model of craniocerebral injury with hydraulic impingement. The water content of brain tissue was measured by dry-wet mass method and Nrf2 cytoplasm, nucleoprotein and HO-1 NQO-1 protein were determined by Western blot. The expression of Nrf2 mRNA was measured by real-time fluorescence quantitative PCR. Results after 6 hours of blast injury, the water content of brain tissue began to increase to the peak for 24 hours and lasted until 3 days. Histological observation confirmed the tendency of brain edema. Western blot analysis showed that cytoplasmic Nrf2 protein continued to decrease 12 hours after injury. However, the nuclear Nrf2 protein began to increase at 1 h and reached the peak at 24 h, then decreased after 7 days. But still higher than NC group (P0.05). The expression of HO-1 and NQO-1 protein was consistent with the trend of nuclear Nrf2 protein. Real-time quantitative PCR showed no significant change in Nrf2 mRNA expression at all time points after TBI (P < 0.05). Conclusion the nuclear factor Nrf2 translocation is activated after hydraulic impact injury. It may play a neuroprotective role by upregulating the expression of HO-1 and NQO-1 protein.
【作者单位】：河北医科大学第二医院急诊科;河北医科大学第二医院神经外科;
【基金】：河北省自然科学基金(C2008001084,H2014206383) 河北省高层次人才资助项目(A201401041) 河北省卫生厅资助课题(20120073,20130178)~~
【分类号】：R651.15
【正文快照】： Surpported by the Natural Science Foundation of Hebei Province(C2008001084,H2014206383),the Project for High Talents of Hebei Prouince(A201401041)创伤性颅脑损伤(traumatic brain injury,TBI)是严重威胁人类健康特别是中青年人群的重要疾病之一,脑外伤后所诱发的

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