抑肽酶对跟腱断裂白兔模型跟腱修复中转化生长因子-β2表达的影响

发布时间：2018-02-14 19:41

本文关键词： 抑肽酶跟腱断裂转化生长因子-β　出处：《中国老年学杂志》2016年21期 　论文类型：期刊论文

【摘要】：目的观察抑肽酶对白兔跟腱断裂修复的影响。方法选用60只新西兰白兔,随机分为三组(正常对照组、模型损伤组、抑肽酶治疗组),于造模后第3天及第1、2、3、4周采集标本,RT-PCR方法检测转化生长因子-β2(TGF-β2)mRNA表达,免疫组化染色观察TGF-β2蛋白表达。结果抑肽酶组在3 d TGF-β2 mRNA表达明显高于模型组(P0.05),在1 w后,抑肽酶组TGF-β2 mRNA表达低于模型组,但无统计学意义。在第2~3周时,抑肽酶组中表达量明显低于模型组(P0.05)。TGF-β2蛋白在正常白兔跟腱有少量表达,术后第3天时抑肽酶治疗组表达量高于模型组,第1周抑肽酶组中表达量明接近模型。2~4 w时抑肽酶组中表达量明显减少且低于模型组。结论抑肽酶可减轻炎症反应,早期可通过调控TGF-β2的表达量,促进跟腱断裂的修复。
[Abstract]:Objective to observe the effect of aprotinin on the repair of Achilles tendon rupture in rabbits. Methods 60 New Zealand white rabbits were randomly divided into three groups (normal control group, model injury group). In the aprotinin treatment group, the expression of TGF- 尾 2 mRNA was detected by RT-PCR on the 3rd day and 4th week after the model, and the expression of TGF- 尾 2 protein was observed by immunohistochemical staining. Results the expression of TGF- 尾 2 mRNA in the aprotinin group was significantly higher than that in the model group on the 3rd day, and after 1 week, the expression of TGF- 尾 2 mRNA in the aprotinin group was significantly higher than that in the model group, and the expression of TGF- 尾 2 mRNA in the aprotinin group was significantly higher than that in the model group. The expression of TGF- 尾 2 mRNA in aprotinin group was lower than that in the model group, but there was no statistical significance. At the 2nd week, the expression of TGF- 尾 2 protein in the aprotinin group was significantly lower than that in the model group, and the expression of TGF- 尾 2 protein in the normal rabbit Achilles tendon was a little lower than that in the model group. On the third day after operation, the expression of aprotinin in the aprotinin group was higher than that in the model group, and the expression level in the aprotinin group was close to that in the model group at the 1st week. The early stage can promote the repair of Achilles tendon rupture by regulating the expression of TGF- 尾 2.
【作者单位】：吉林省人民医院;
【基金】：吉林省自然科学基金项目(No.201215199)
【分类号】：R-332;R686.1

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