脓毒血症引起肺损伤的实验研究

发布时间：2018-02-25 01:14

本文关键词： 脓毒血症肺损伤肿瘤坏死因子-α 白细胞介素-　出处：《广东医学》2017年S2期 　论文类型：期刊论文

【摘要】：目的探讨脓毒血症引起肺损伤的相关机制。方法将30只SD大鼠随机均分为正常对照组和模型组,1周后分别予等体积的生理盐水、8 mg/kg的脂多糖(LPS)尾静脉注射,48 h后取支气管肺泡灌洗液(BALF)和肺组织,测定BALF中的中性粒细胞计数;ELISA法测定肺组织和BALF中肿瘤坏死因子-α(TNF-α)及白细胞介素-6(IL-6)的浓度,称重并计算肺组织湿/干重比(W/D)。观察小鼠肺组织病理切片并行肺损伤评分。结果与正常对照组比较,模型组小鼠肺泡组织结构发生明显改变,大鼠肺组织肿大,有轻微的溃烂,且肺组织W/D较正常对照组显著增加,HE染色显示模型组组织膨胀,细胞水肿疏松,并有部分细胞脱落,肺损伤评分明显升高(P0.01),正常对照组无明显病变。模型组BALF中的中性粒细胞计数显著提高(P0.01),肺组织和BALF中TNF-α和IL-6浓度显著升高(P0.01)。结论脓毒血症肺组织、BALF炎性因子TNF-α和IL-6明显升高,脓毒血症所致急性肺损伤的病变程度加重,中性粒细胞的浸润、肺组织炎症介质的产生和渗出增加。脓毒血症可引起的肺水肿和大分子物质渗出。
[Abstract]:Objective to investigate the mechanism of lung injury induced by sepsis. Methods Thirty SD rats were randomly divided into normal control group and model group after injection of the same volume of normal saline for 8 mg/kg of lipopolysaccharide (LPS) into tail vein for 48 h. Bronchoalveolar lavage fluid (BALF) and lung tissue, The concentrations of tumor necrosis factor- 伪 (TNF- 伪) and interleukin-6 (IL-6) in lung tissue and BALF were determined by Elisa. The lung tissue wet / dry weight ratio was measured and the lung tissue pathological sections were observed with lung injury score. Results compared with the normal control group, the alveolar tissue structure of the model group was obviously changed, the lung tissue in the model group was enlarged, and the lung tissue was slightly ulcerated. Compared with the normal control group, the lung tissue W- / D increased significantly with HE staining, which showed that the model group had tissue swelling, cell edema and loose, and some of the cells were exfoliated. In model group, the neutrophil count in BALF and the concentrations of TNF- 伪 and IL-6 in lung tissue and BALF were significantly increased (P 0.01). Conclusion the inflammatory cytokines TNF- 伪 and IL-6 in septic lung tissue were significantly higher than those in control group. The severity of acute lung injury due to sepsis is aggravated, neutrophils infiltrate, and the production and exudation of inflammatory mediators in lung tissue are increased.
【作者单位】：深圳市急救中心;深圳市第二人民医院急诊病房;
【基金】：深圳市科技创新委员会知识创新计划基础研究项目类资助项目(编号:JCYJ20140416150012350)
【分类号】：R459.7

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