大鼠颅脑损伤后突触素表达变化研究

发布时间：2018-02-25 17:17

本文关键词： 颅脑损伤动物模型影像学组织病理学　出处：《四川大学学报(医学版)》2016年06期 　论文类型：期刊论文

【摘要】：目的探讨大鼠颅脑损伤后突触素变化规律并探索其与组织学、影像学的动态变化关系。方法健康3月龄雄性SD大鼠24只,依据突触素检测时间,分为损伤后2周、4周、8周组和对照组共4组,每组6只。其中3个损伤组通过建立大鼠自由落体脑损伤模型,在损伤后24h、2周、4周和8周时通过CT检查观察其颅脑损伤后脑组织的影像学变化,对照组不作损伤处理。同时检测大鼠颅脑损伤后脑组织形态学及影像学变化,并分别于2周、4周、8周使用免疫荧光法及蛋白免疫印迹实验观察受伤后大鼠脑组织突触素表达变化。结果大鼠颅脑损伤后,神经功能评分提示实验组大鼠出现明显神经功能障碍,HE染色提示实验组大鼠受伤24h出现脑细胞水肿、坏死。另外尚可见局部的充血,受伤后2周后出现脑溶解性液化及坏死,而受伤4周及8周后除可见明显组织缺损外,无明显组织学变化。CT检查提示实验组大鼠术后24h呈现全脑明显的低密度影,此后脑组织低密度灶的区域及程度开始减轻,至受伤后2周低密度影已经明显减退,受伤后4周及8周时脑损伤已经不明显,仅可见骨窗缺损。免疫荧光染色及western blot均提示突触素表达于2周下降,第8周略有上升。结论成功建立了Feeney大鼠自由落体脑损伤模型。大鼠颅脑损伤后,突触素在受损急性期呈现下降,持续至脑损伤后第8周开始恢复。突触素的表达随时间变化的规律与影像学、组织病理学变化有关。
[Abstract]:Objective to investigate the changes of synaptophysin in rats after craniocerebral injury and to explore the dynamic relationship between synaptophysin and histology and imaging. Methods 24 healthy male Sprague-Dawley rats aged 3 months were used to detect synaptophysin according to the time of synaptophysin detection. The rats were divided into 4 groups, 6 rats in each group, 2 weeks after injury and 8 weeks after injury. The imaging changes of brain tissue after brain injury were observed by CT at 2 weeks and 8 weeks after the injury, but not in the control group. At the same time, the morphologic and imaging changes of brain tissue were detected after craniocerebral injury in rats. The changes of synaptophysin expression in rat brain tissue were observed by immunofluorescence assay and Western blot assay at 2 weeks and 4 weeks and 8 weeks, respectively. Results after craniocerebral injury, the expression of synaptophysin was observed in rats. The neurological function score indicated that the rats in the experimental group had obvious neurological dysfunction and HE staining suggested that the rats in the experimental group had edema and necrosis of brain cells at 24 h after injury, and local hyperemia was also observed, and the brain dissolving liquefaction and necrosis occurred 2 weeks after injury. But after 4 and 8 weeks of injury, there was no obvious histological change except obvious tissue defect. Ct examination showed that the brain of the experimental group showed obvious low density image 24 hours after operation, and the area and degree of the low density focus of brain tissue began to lighten after 4 weeks and 8 weeks of injury. By the 2nd week after injury, the low density shadow had obviously decreased, the brain injury was not obvious at 4 and 8 weeks after injury, only bone window defect was observed. Immunofluorescence staining and western blot showed that synaptophysin expression decreased at 2 weeks. Conclusion the free falling brain injury model of Feeney rats was successfully established. The synaptophysin level decreased in the acute stage after craniocerebral injury in rats. The changes of synaptophysin expression over time were related to imaging and histopathological changes.
【作者单位】：四川大学华西医院神经外科;成都大学附属医院神经外科;四川省第二人民医院病理科;
【基金】：国家自然科学基金(No.81100925,No.81472361)资助
【分类号】：R651.15

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