急性肝衰竭小鼠线粒体分裂蛋白DRP1在肝脏和大脑皮层的表达变化

发布时间：2018-02-28 06:32

本文关键词： 急性肝衰竭线粒体 DRP 肝脏皮层小鼠　出处：《神经解剖学杂志》2017年03期 　论文类型：期刊论文

【摘要】：目的:观察C57小鼠急性肝衰竭(acute liver failure,ALF)过程中线粒体分裂蛋白(dynamic-related protein 1,DRP1)在肝脏和大脑皮层中的变化及与肝衰竭的相关性。方法:C57/BL小鼠,随机分为对照组、ALF1d、4 d、7 d组。腹膜腔内注射硫代乙酰胺(TAA)建立ALF模型,利用高架十字和旷场实验测定行为学,取血清检测谷氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)水平;之后取肝组织,HE染色观察肝组织的病理变化;用Western Blot和RT-q PCR检测DRP1在肝脏和大脑皮层中蛋白及mRNA水平的表达变化。结果:(1)行为学结果显示:ALF各组小鼠自发活动及探索行为均明显降低(P0.05);(2)肝功检测ALF组ALT、AST表达水平明显升高(P0.05);(3)肝组织病理学检查ALF组1 d时肝细胞出现大面积坏死,7 d时肝细胞坏死较1 d、4 d缓解;(4)Western Blot检测DRP1在ALF全肝组织和肝线粒体中明显降低(P0.05);而在大脑皮层全组织蛋白中明显升高(P0.05),但在提取的线粒体中则无明显改变(P0.05);(5)RT-q PCR检测DRP1在肝组织中ALF各组中表达明显降低(P0.05);而在大脑皮层组织中1 d时表达增多,持续到4 d,在7 d时恢复(P0.05)。结论:DRP1在急性肝衰竭小鼠的肝脏和大脑皮层中对线粒体形态学发挥着重要的作用。
[Abstract]:Objective: to observe the changes of mitochondrial mitotic protein 1 (DRP1) in the liver and cerebral cortex of C57 mice during acute liver failure and its correlation with liver failure. ALF model was established by intraperitoneal injection of thioacetamide (TAA). Behavior was measured by elevated cross and open field experiments. Serum levels of glutamate aminotransferase (alt) and aspartate transaminase (AST) were measured in serum. The pathological changes of liver tissue were observed by HE staining. Western Blot and RT-q PCR were used to detect the expression of DRP1 protein and mRNA in the liver and cerebral cortex. Results the behavioral results showed that the spontaneous activity and exploring behavior of mice in the groups of Western Blot and RT-q decreased significantly (P 0.05). Histopathological examination of liver histopathology in ALF group, large area necrosis of hepatocytes was found on day 1 and DRP1 was significantly decreased in whole liver tissue and hepatic mitochondria of ALF at 7 days and 4 days after treatment compared with 4 days after 1 day, while in the cortex of cerebrum, it was significantly lower than that of DRP1 in the whole liver tissue and mitochondria of the liver. The expression of P0.05 in the whole tissue protein was significantly increased, but there was no significant change in the extracted mitochondria. The expression of DRP1 in the liver tissue decreased significantly in the ALF group, but increased in the cerebral cortex tissue at 1 day. It lasted until 4 days, and recovered to P0.05 at 7 days. Conclusion: DRP1 plays an important role in the morphology of mitochondria in the liver and cerebral cortex of mice with acute liver failure.
【作者单位】：第四军医大学人体解剖与组织胚胎学教研室暨梁挾琚脑研究中心;第四军医大学西京医院肝胆胰脾外科;
【基金】：国家自然科学基金(81272555,81470843)
【分类号】：R575.3

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