阿替普酶对急性脑梗死大鼠血管内皮细胞中Claudin-1和Claudin-5蛋白表达的影响

发布时间：2018-02-28 21:32

本文关键词： 阿替普酶急性脑梗死血管内皮细胞闭合蛋白闭合蛋白　出处：《吉林大学学报(医学版)》2017年06期 　论文类型：期刊论文

【摘要】：目的:探讨阿替普酶对急性脑梗死大鼠溶栓后血管内皮细胞中闭合蛋白1(Claudin-1)和闭合蛋白5(Claudin-5)表达的影响及保护作用机制,为阿替普酶的临床应用提供依据。方法:建立大鼠急性大脑中动脉栓塞模型。54只SD大鼠随机分为假手术组、模型组和阿替普酶溶栓组,每组18只。透射电子显微镜下观察大鼠脑内皮细胞超微结构,荧光免疫组织化学法和Western blotting法检测大鼠血管内皮细胞中Claudin-1和Claudin-5蛋白的表达水平。结果:透射电子显微镜检测,模型组大鼠缺血区域脑体积明显增大,内皮细胞肿胀,部分皮质与周围脑组织界限明显,基膜厚薄均匀,紧密连接结构非常松散、出现断裂和消失;阿替普酶溶栓组大鼠脑梗死区域毛细血管内皮细胞肿胀明显减轻,基膜厚薄不均匀改善,脑毛细血管内皮细胞、内皮细胞之间大部分紧密连接结构断裂情况消失,无紧密连接结构消失。荧光免疫组织化学检测,与模型组比较,阿替普酶溶栓组大鼠血管内皮细胞中Claudin-1和Claudin-5蛋白的表达有明显改善。Western blotting检测,与假手术组比较,模型组大鼠血管内皮细胞中Claudin-1和Claudin-5蛋白表达水平明显减少(P0.01);与模型组比较,不同时间点阿替普酶溶栓组大鼠血管内皮细胞中Claudin-1和Claudin-5蛋白表达水平均升高(P0.01)。结论:阿替普酶对急性脑梗死大鼠大脑内皮细胞的结构有改善作用,其作用机制可能与阿替普酶能降低大鼠血管内皮细胞中Claudin-1和Claudin-5蛋白表达水平有关。
[Abstract]:Objective: to investigate the effect of atropase on the expression of closure protein 1 Claudin-1 and closure protein 5 Claudin-5 in vascular endothelial cells of rats with acute cerebral infarction after thrombolytic therapy and its protective mechanism. Methods: 54 SD rats with acute middle cerebral artery embolization were randomly divided into sham-operation group, model group and atropase thrombolytic group. The ultrastructure of rat brain endothelial cells was observed under transmission electron microscope, and the expression of Claudin-1 and Claudin-5 protein in rat vascular endothelial cells was detected by fluorescence immunohistochemical method and Western blotting method. Results: transmission electron microscopy was used to detect the expression of Claudin-1 and Claudin-5 protein in rat vascular endothelial cells. In the model group, the cerebral volume of ischemic area was obviously enlarged, the endothelial cells were swollen, the boundary between some cortex and peripheral brain tissue was obvious, the thickness of basement membrane was uniform, the tight junction structure was very loose, and the rupture and disappearance occurred. The swelling of capillary endothelial cells in the cerebral infarction area was obviously reduced, the thickness of the basement membrane was improved inhomogeneously, and most of the tight junctions between the cerebral capillary endothelial cells and the endothelial cells disappeared in the AtiP thrombolytic group. Compared with the model group, the expression of Claudin-1 and Claudin-5 protein in the vascular endothelial cells of the rats treated with atropase thrombolytic therapy was significantly improved. Western blotting was detected, and compared with the sham-operated group, the expression of Claudin-1 and Claudin-5 protein in the vascular endothelial cells of the rats treated with atropase thrombolytic therapy was significantly improved. The expression of Claudin-1 and Claudin-5 protein in vascular endothelial cells of the model group was significantly decreased compared with the model group. At different time points, the expression of Claudin-1 and Claudin-5 protein in vascular endothelial cells were increased in rats treated with atropase at different time points. Conclusion: Atiprase can improve the structure of cerebral endothelial cells in rats with acute cerebral infarction. The mechanism may be related to the decrease of Claudin-1 and Claudin-5 protein expression in rat vascular endothelial cells by atropase.
【作者单位】：承德医学院附属医院神经内科;
【基金】：河北省科技厅科研项目资助课题(15277752D) 河北省承德市科技局自筹项目资助课题(20157044)
【分类号】：R743.33

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