单纯性脑震荡对大鼠海马iNOS表达影响的实验研究

发布时间：2018-03-04 14:01

本文选题：单纯性脑震荡　切入点：海马　出处：《中国临床解剖学杂志》2016年04期 　论文类型：期刊论文

【摘要】：目的观察单纯性脑震荡(pure cerebral concussion,PCC)大鼠海马脑区诱导性一氧化氮合酶(inducible nitric oxide synthase,i NOS)的表达变化,探讨i NOS表达变化规律及其与PCC之间的病理联系。方法采用单摆闭合式脑损伤打击装置制造PCC大鼠模型,造模后随机分为3 h、12 h、1 d、2 d、3d、7 d六个损伤组(n=5),另设正常对照组(n=5)。采用i NOS单克隆抗体(鼠)进行免疫组织化学SP法染色和Western Blot实验,检测PCC组和正常组大鼠海马(CA1~4及上、下齿状回)i NOS表达的定位及定量情况。结果正常生理状态下,海马i NOS阳性细胞染色较浅、轮廓不清,且i NOS蛋白表达较弱,致伤后3 h组i NOS阳性表达开始增强,在3 d组出现表达高峰,i NOS蛋白表达高峰出现在2 d组,与正常组比较均有显著性差异(P0.05),随后均出现下降,至7 d均仍高于正常组。结论 PCC损伤早期海马脑区出现i NOS表达增强,提示i NOS是参与PCC继发性病理损伤的主要炎症因子之一。
[Abstract]:Objective to observe the expression of inducible nitric oxide nitric oxide synthase (NOS) in the hippocampus of pure cerebral concussionPCC rats. To investigate the changes of I NOS expression and the pathological relationship between I NOS and PCC. Methods the rat model of PCC was established by using a single pendulum closed brain injury device. After modeling, the rats were randomly divided into six injury groups (3 h, 12 h, 1 day, 2 d, 3 d and 7 d), and the normal control group was set up. Immunohistochemical SP staining with I NOS monoclonal antibody (mouse) and Western Blot assay were used to detect CA14 and its level in hippocampus of rats in PCC group and normal group. Results under normal physiological conditions, I NOS positive cells in hippocampus were less stained, and the expression of I NOS protein was weak. The positive expression of I NOS began to increase 3 hours after injury. After 3 days, the peak expression of NOS protein was found in the 2 day group, which was significantly different from that in the normal group (P 0.05), and then decreased. Conclusion the expression of I NOS in hippocampal area is increased in the early stage of PCC injury, suggesting that I NOS is one of the main inflammatory factors involved in the secondary pathological injury of PCC.
【作者单位】：昆明医科大学人体解剖学与组织胚胎学系;齐鲁医药学院人体解剖学与组织胚胎学教研室;西安交通大学医学部法医学院;河南护理职业学院人体解剖学与组织胚胎学系;昆明医科大学第一附属医院急诊医学科;昆明医科大学法医学院;昆明医科大学脑损伤研究室;
【基金】：国家自然科学基金资助项目(31100769,30560041) 云南省自然科学基金(2008CD052)
【分类号】：R651.15

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