腱止点过度使用性损伤组织:形态学及生长因子的变化

发布时间：2018-03-06 10:06

本文选题：累积创伤　切入点：成纤维细胞生长因子　出处：《中国组织工程研究》2017年08期 　论文类型：期刊论文

【摘要】：背景:髌骨髌腱结合部过度使用性损伤十分常见,目前学术界对该病的发病及愈合机制尚不清楚。目的:拟通过对髌腱止点处进行反复牵拉建立过度使用性损伤动物模型,从组织形态学及生长因子变化角度探讨过度使用性损伤及愈合的机制。方法:28只新西兰大白兔随机分为4组,空白对照组4只,左右后肢均作为对照;损伤造模组、自然愈合组和低强度干预组各8只,左后肢进行循环载荷训练。损伤造模组训练4周后立即处死;自然愈合组训练4周后继续休息4周处死;低强度干预组训练4周后改换低强度继续训练4周;对照组不做任何处理。观察循环载荷训练对兔髌骨髌腱结合部损伤形成及愈合过程中组织形态学、生长因子表达量的影响。结果与结论:(1)与空白对照组相比,损伤造模组细胞排列紊乱,潮线消失,胶原纤维波浪状结构消失;自然愈合组及低强度干预组织学形态虽未能恢复正常但较损伤造模组更好;(2)3个实验组的纤维软骨带厚度及碱性成纤维细胞生长因子均显著高于空白对照组(P0.05);损伤造模组细胞浓度及血管内皮生长因子明显高于空白对照组和自然愈合组(P0.05);(3)结果表明,髌骨髌腱结合部过度使用性损伤造模成功后,休息4周仍不能恢复到正常状态,而伤后进行低强度训练有利于改善愈合质量。
[Abstract]:Background: overuse injury of patella patellar tendon junction is very common, and the pathogenesis and healing mechanism of patellar tendon junction are not clear in academic circles. Objective: to establish the animal model of overuse injury by repeatedly pulling the patellar tendon junction. Methods Twenty eight New Zealand white rabbits were randomly divided into four groups, the blank control group (n = 4), the left and right hind limbs as control group, and the injury model group. In the natural healing group and the low intensity intervention group, the left hind limb was trained by cyclic load training, the injury model group was executed immediately after 4 weeks of training, and the natural healing group was executed after 4 weeks of training and rest for 4 weeks. The low-intensity intervention group changed the low-intensity training for 4 weeks, and the control group did not do any treatment. The tissue morphology of patellar tendon injury and healing in rabbit patellar tendon was observed by cyclic load training, and the effect of cyclic load training on the formation and healing of patella patellar tendon injury was observed. Results compared with the control group, the cells of the model group were disordered, the tidal lines disappeared, and the wave structure of collagen fibers disappeared. The thickness of fibrous chondrocytes and basic fibroblast growth factor in the three experimental groups were significantly higher than those in the control group (P 0.05). The cell concentration and vascular endothelial growth factor (VEGF) in the model group were significantly higher than those in the control group and the natural healing group (P 0.05). After the patellar patellar tendon joint overuse injury was established successfully, the rest for 4 weeks could not return to the normal state, but the low intensity training after the injury was helpful to improve the healing quality.
【作者单位】：北京体育大学运动康复系;北京体育大学运动医学教研室;
【基金】：国家自然科学基金课题(30871209)~~
【分类号】：R686

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