脂肪间充质干细胞治疗外伤性脑损伤

发布时间：2018-03-10 11:39

本文选题：间充质干细胞　切入点：干细胞移植　出处：《中国组织工程研究》2017年01期 　论文类型：期刊论文

【摘要】：背景:临床与动物研究证实,间充质干细胞移植能迁移至脑损伤区域,对创伤性脑损伤有一定的治疗效果。目的:进一步验证脂肪间充质干细胞对大鼠外伤性脑损伤造成的局部损伤的治疗作用。方法:大鼠随机分为3组:实验组和对照组参照文献以脑冷冻伤法制作SD大鼠脑损伤模型,实验组造模成功2 d后进行脂肪间充质干细胞尾静脉移植;对照组只尾静脉注射等量生理盐;正常组大鼠不作任何处理。采用Morris水迷宫测试评价大鼠神经功能恢复情况;体个分离、培养脂肪间充质干细胞,在倒置显微镜下观察脂肪间充质干细胞的形态,免疫组织化学检测干细胞在大鼠损伤脑组织中的分布及脑源性神经营养因子和胶质细胞源性神经营养因子含量。结果与结论:(1)Morris水迷宫测试结果显示:与对照组比,实验组SD大鼠平均逃避潜伏期迅速下降(P0.05),而同时实验组大鼠逃避潜伏期越来越接近于正常组大鼠;(2)免疫组织化学后倒置显微镜观察:移植的经Brdu标记的脂肪间充质干细胞大量聚集在损伤的大脑皮质,呈不均匀分布。而对照组大鼠脑组织内未见Brd U免疫荧光显色;(3)Western-blot检测结果显示:实验组经脂肪干细胞静脉移植后,SD大鼠海马区域中的脑源性神经营养因子和周围损伤皮质中的胶质细胞源性神经营养因子的含量明显高于对照组(P0.05)。而与正常组SD大鼠比较,对照组大鼠脑组织中海马区域中的脑源性神经营养因子含量升高(P0.05),而皮质周围的胶质细胞源性神经营养因子含量却明显下降(P0.05);(4)实验结果提示移植的脂肪间充质干细胞向大鼠脑组织的损伤部位分布,并促进了受损脑组织的胶质细胞源性神经营养因子和脑源性神经营养因子分泌,这也可能是对大鼠神经功能恢复的治疗促进作用之一。
[Abstract]:Background: clinical and animal studies have confirmed that mesenchymal stem cell transplantation can migrate to brain injury areas. Objective: to further verify the therapeutic effect of adipose mesenchymal stem cells on local injury caused by traumatic brain injury in rats. Methods: rats were randomly divided into three groups: experimental group and experimental group. In the control group, the brain injury model of SD rats was made by using the method of brain freezing injury according to the literature. The rats in the experimental group were transplanted with adipose mesenchymal stem cell caudal vein 2 days later, the control group was injected with the same amount of physiological salt only through the tail vein, the normal group rats were not treated. The Morris water maze test was used to evaluate the recovery of nerve function in the rats. The adipose mesenchymal stem cells were isolated and cultured, and the morphology of adipose mesenchymal stem cells was observed under inverted microscope. The distribution of stem cells in injured brain tissue and the contents of brain-derived neurotrophic factor and glial cell-derived neurotrophic factor were detected by immunohistochemistry. The average escape latency of SD rats in the experimental group decreased rapidly (P 0.05), while the escape latency in the experimental group was more and more close to that in the normal group. The stem cells accumulate in large numbers in the damaged cortex of the brain. The results of Western-blot analysis showed that brain derived neurotrophic factor (BDNF) and peripheral neurotrophic factor (BDNF) in the hippocampus of SD rats were found in the experimental group after vein transplantation of adipose stem cells. The content of glial cell derived neurotrophic factor (GDNF) in injured cortex was significantly higher than that in control group (P 0.05). In control group, the content of brain-derived neurotrophic factor (BDNF) in hippocampal region of the control group was increased, while the content of glial cell-derived neurotrophic factor (GDNF) around the cortex decreased significantly (P0.05). The results suggest that the transplanted adipose mesenchymal stem was transplanted. The cells were distributed to the injured site of brain tissue in rats. It also promotes the secretion of glial cell-derived neurotrophic factor and brain-derived neurotrophic factor in injured brain tissue, which may be one of the therapeutic effects to promote the recovery of nerve function in rats.
【作者单位】：河北医科大学附属唐山工人医院;
【分类号】：R394.2

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