旋毛虫及其虫源性蛋白对盲肠结扎穿孔诱导的小鼠哝毒症的影响

发布时间：2018-03-11 12:54

本文选题：脓毒症　切入点：旋毛虫　出处：《南方医科大学学报》2016年08期 　论文类型：期刊论文

【摘要】：目的观察旋毛虫及其虫源性蛋白对盲肠结扎穿孔(CLP)诱导的小鼠脓毒症的影响。方法 80只雄性BALB/c小鼠随机分为假手术组、CLP组、旋毛虫肌幼虫(ML)预感染组、旋毛虫肌幼虫虫体可溶性蛋白(SMP)处理组和排泄分泌蛋白(MES)处理组。ML预感染组于术前28 d经口感染300条旋毛虫肌幼虫,其余各组分别于术后30 min腹腔注射PBS或SMP(25μg/只)或MES(25μg/只)。观察小鼠术后状态和72 h生存率,检测术后12 h小鼠血清中丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、尿素氮(BUN)和肌酐(Cr)水平及TNF-α、IL-6、IL-1β、IL-10、TGF-β水平,观察小鼠肝和肾组织病变。结果与假手术组相比,CLP组72 h生存率降低,血清中ALT、AST、BUN和Cr水平及细胞因子TNF-α、IL-6、IL-1β、IL-10和TGF-β水平均明显升高(P0.05)。肝中肝索排列紊乱,肝细胞水肿,肾中部分血管球皱缩,肾小管细胞水肿。与CLP组相比,ML预感染组血清中ALT、AST、Cr、TNF-α和IL-1β水平降低,IL-10和TGF-β水平升高(P0.05);SMP处理组血清中ALT、AST、Cr、TNF-α和IL-1β水平降低,TGF-β水平升高(P0.05);MES处理组72 h生存率明显升高,血清中ALT、AST、BUN、Cr、TNF-α、IL-6和IL-1β水平明显降低,IL-10和TGF-β水平明显升高(P0.05),肝和肾组织结构损伤明显减轻。结论旋毛虫及其虫源性蛋白可减少CLP诱导的脓毒症小鼠血清中促炎因子的释放,促进免疫调节因子的释放,其中MES效果更为显著,并能减轻肝和肾结构和功能的损伤。
[Abstract]:Objective to observe the effect of Trichinella spiralis and its insect-derived protein on sepsis induced by cecal ligation and perforation in mice. Methods 80 male BALB/c mice were randomly divided into two groups: sham operation group and trichinella muscle larva preinfection group. The muscle larvae of Trichinella spiralis were treated with soluble protein (SMPP) and excretory and secretory protein (MESs). ML preinfection group was infected with 300 trichinella muscle larvae by mouth 28 days before operation. The other groups were injected intraperitoneally with PBS or SMP(25 渭 g / mouse or MES(25 渭 g / mouse at 30 min after operation. The postoperative state and 72 h survival rate of the mice were observed. The serum levels of alanine aminotransferase (alt), aspartate aminotransferase (ASTU), bun (bun), creatinine (Cr) and TNF- 伪 IL-6 尾 IL-10 TGF- 尾 in serum of mice were measured 12 hours after operation, and the pathological changes of liver and kidney were observed. Results the 72 h survival rate of CLP group was lower than that of sham CLP group. The levels of bun and Cr in serum and cytokines TNF- 伪, IL-6, IL-1 尾, IL-10 and TGF- 尾 in serum were significantly higher than those in control group (P 0.05). Renal tubular cell edema. Compared with CLP group, the serum levels of TNF- 伪 and TGF- 尾 in ML-preinfection group were decreased, the levels of IL-10 and TGF- 尾 were increased, the serum levels of TGF- 伪 and TGF- 尾 in serum of P0.05- SMPtreated group increased significantly, and the survival rate of TGF- 尾 increased significantly in P0.05MES treatment group. The levels of TNF- 伪 IL-6 and IL-1 尾 in serum of Asthma sinensis were significantly decreased, the levels of IL-10 and TGF- 尾 increased significantly, and the damage of liver and kidney tissue structure was alleviated. Conclusion Trichinella spiralis and its insect-derived protein can reduce the release of pro-inflammatory factor in serum of CLP induced sepsis mice. Promote the release of immunomodulators, MES is more effective, and can reduce the damage of liver and kidney structure and function.
【作者单位】：蚌埠医学院组织学与胚胎学教研室;蚌埠医学院病原生物学教研室;蚌埠医学院病理学教研室;蚌埠医学院第二附属医院;蚌埠医学院人体解剖学教研室;
【基金】：国家自然科学基金项目(81441120) 高校科研创新平台团队项目(2016-40) 安徽省自然科学基金项目(1508085QH158) 安徽省高校优秀青年人才支持计划重点项目(gxyq ZD2016159) 安徽省高等学校自然科学研究重点项目(KJ2016A708) 安徽省自然科学青年科学基金项目(1608085QC51) 安徽省高等学校自然科学研究项目(KJ2015B013by) 蚌埠医学院自然科学研究重点项目(BYKL1402ZD) 安徽省级大学生创新项目(201510367016)~~
【分类号】：R459.7

【相似文献】