三种方法复制“二次打击”急性呼吸窘迫综合征动物模型的比较与评价

发布时间：2018-03-12 12:48

本文选题：急性呼吸窘迫综合征　切入点：“二次打击”模型　出处：《中国实验动物学报》2017年03期 　论文类型：期刊论文

【摘要】：目的通过研究三种不同"二次打击"急性呼吸窘迫综合征(acute respiratory distress syndrome,ARDS)动物模型的特点,寻找较为理想的ARDS动物模型。方法将48只大鼠随机分为4组,每组12只。对照组(Control组):尾静脉先注射生理盐水(normal saline,NS)2.5 m L/kg,30 min后注射NS 2.5 m L/kg;油酸+油酸组(OA+OA组):尾静脉先注入OA 0.05 m L/kg,30 min后继续注射OA 0.5 m L/kg;内毒素+内毒素组(LPS+LPS组):尾静脉先注入LPS 2.5 mg/kg,30 min后继续注射LPS 2.5 mg/kg;油酸+内毒素组(OA+LPS组):尾静脉先注入OA 0.05 m L/kg,30 min后注射LPS 2.5 mg/kg。5 h后处死动物采集标本,检测动脉血气,支气管肺泡灌洗液(bronchoalveolar lavage fluid,BALF)中白细胞总数、多形核白细胞百分比(polymorphonuclear leukocyte ratio,PMN%)、肿瘤坏死因子(tumor necrosis factorα,TNF-α)水平、总蛋白含量,肺干湿重比(lung wet-dry weight ratio,W/D),观察肺组织病理改变并进行肺损伤评分。结果与control组比较,LPS+LPS组、OA+OA组及OA+LPS组的氧分压(Pa O2)下降(P0.05)、二氧化碳分压(Pa CO2)、BALF中白细胞总数、PMN%、TNF-α、总蛋白含量,W/D、肺损伤评分均升高(P0.05)。OA+LPS组总蛋白含量、W/D均高于LPS+LPS组,差异有显著性(P0.05),而与OA+OA组比较则差异无显著性;OA+LPS组BALF中白细胞计数、PMN%、TNF-α较OA+OA组高(P0.05),而与LPS+LPS组比较则差异无显著性;OA+LPS组肺病理改变最典型,肺损伤评分最高。结论 OA+OA、LPS+LPS和OA+LPS复合打击法均可建立"二次打击"ARDS大鼠模型,其中OA+LPS复合打击法所复制的ARDS模型与临床ARDS特点更为接近,有利于研究ARDS的病理生理过程,是一种较为理想的"二次打击"ARDS动物模型。
[Abstract]:Objective to study the characteristics of three different "second strike" acute respiratory distress syndrome (ARDS) animal models and to find an ideal ARDS animal model. Methods 48 rats were randomly divided into 4 groups. 12 rats in each group. Control group: caudal vein injection of normal saline NS2.5 mL / kg for 30 min and then injection of NS 2.5 mL / kg for 30 min; oleic acid group for OA OA group: caudal vein injection of OA 0.05 mL / kg for 30 min followed by injection of OA 0.5 mL / kg; endotoxin; endotoxin. In LPS group, the caudal vein was injected with LPS 2.5 mg / kg for 30 min and then LPS 2.5 mg / kg after 30 min. In the oleic acid endotoxin group, the OA LPS group was injected with OA 0.05ml 路kg ~ (-1) / kg ~ (30) min first, and then the animals were killed after injection of LPS 2.5 mg/kg.5 for 2. 5 h. The arterial blood gas, the total number of leukocytes in bronchoalveolar lavage (BALF), the percentage of polymorphonuclear leukocyte neutrophil (PMN- 伪), the level of tumor necrosis factor 伪 (TNF- 伪) and the content of total protein in bronchoalveolar lavage (BALF- 伪) of bronchoalveolar lavage fluid (BALF) were measured. Lung wet-dry weight ratio W / D, lung tissue pathological changes were observed and lung injury scores were evaluated. Results compared with control group, the oxygen partial pressure (PO2) in control LPS group and OA LPS group decreased (P0.05N), and the total number of white blood cells (PMNN- 伪) in control LPS group and OA LPS group decreased (P < 0.05), and the total number of PMNN- 伪 in control group was compared with that in control group. The white content and lung injury score were all higher in OA LPS group than in LPS LPS group, and the total protein content in OA LPS group was higher than that in LPS LPS group. There was no significant difference in leukocyte count in BALF between OA LPS group and OA OA group (P 0.05), but there was no significant difference compared with LPS LPS group (P 0.05), but there was no significant difference between LPS LPS group and OA LPS group in lung pathological changes. The leukocyte count in BALF of OA LPS group was higher than that in OA OA LPS group (P 0.05), but there was no significant difference compared with LPS LPS group. Conclusion the rat model of "secondary strike" ARDS can be established by combined attack of OA OAA LPS and OA LPS, in which the ARDS model of OA LPS compound attack is more similar to that of clinical ARDS. It is helpful to study the pathophysiological process of ARDS and is an ideal animal model of ARDS.
【作者单位】：广州医科大学基础学院生理学教研室;广州医科大学第三临床学院;广州医科大学第一临床学院;中山大学中山医学院药理学教研室;
【基金】：国家自然科学基金(编号:81302771) 广东省医学科学研究基金(编号:A2016382) 广东省自然科学基金(编号:2014A030313087) 国家级大学生创新创业训练计划项目(编号:2015105700010) 广州医科大学大学生科技创新项目(编号:2014A015)
【分类号】：R-332;R563.8

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