高压氧治疗对创伤性脑损伤大鼠认知功能的影响

发布时间：2018-03-17 16:15

  本文选题：高压氧　切入点：创伤性脑损伤　出处：《中国康复医学杂志》2017年11期 　论文类型：期刊论文

【摘要】：目的:观察高压氧治疗对脑损伤大鼠认知功能的影响及海马区CCL2及其受体CCR2的表达变化。方法:75只成年雄性SD大鼠按数字表法随机分为假手术组(Sham组)、脑外伤组(TBI组)和高压氧治疗组(HBOT组),每组各25只。HBOT组和TBI组均采用Feeney自由落体法制作脑外伤模型,HBOT组每天进行HBO治疗;Sham组暴露硬脑膜不予打击。运用Morris水迷宫测试认知功能;荧光免疫双标检测海马CA1区CCL2和CCR2的表达。实时定量PCR测定损伤侧海马CCL2和CCR2mRNA的表达情况。结果:Morris水迷宫测试结果显示,HBOT组高压氧治疗后7d、14d和21d平均潜伏期下降,同时穿越平台次数增多,与TBI组相比,差异均有显著性意义(P0.05);免疫荧光双染法检测显示,大鼠TBI后海马CA1区CCL2主要表达在星形胶质细胞,CCR2主要表达在神经元;实时定量PCR显示,脑损伤后3—21d损伤侧海马CCL2 mRNA、CCR2 mRNA水平明显上升,差异有显著性意义;高压氧治疗后海马CCL2 mRNA明显下降,与TBI组相比,7d组、14d组及21d组差异有显著性意义(P0.05)。高压氧治疗7d、14d后海马CCR2 mRNA明显下降,与TBI组相比,7d组及14d组差异有显著性意义(P0.05)。结论:HBO治疗可以改善创伤性脑损伤大鼠认知功能,其机制可能与海马CCL2/CCR2表达下调有关。
[Abstract]:Objective: to observe the effect of hyperbaric oxygen therapy on cognitive function and the expression of CCL2 and its receptor CCR2 in hippocampus of rats with brain injury. Methods: 75 adult male SD rats were randomly divided into sham group and brain injury group according to digital table. HBOT group (25 rats in each group) and hyperbaric oxygen treatment group (HBOT group) and TBI group were treated with HBO every day to treat the dura mater exposed to the dura mater. The cognitive function was tested by Morris water maze. The expression of CCL2 and CCR2 in hippocampal CA1 was detected by fluorescence immunoassay, and the expression of CCL2 and CCR2mRNA in injured hippocampus was measured by real-time quantitative PCR. Results the results of water maze test showed that the mean latency of HBOT group was decreased on day 14 and day 21 after hyperbaric oxygen therapy. At the same time, the frequency of crossing the platform increased, compared with the TBI group, the difference was significant (P 0.05). The results of immunofluorescence double staining showed that the expression of CCL2 in the CA1 area of hippocampus was mainly in astrocytes and neurons after TBI, and the real-time quantitative PCR showed that the CCL2 was mainly expressed in the neurons. The level of CCL2 mRNA-CCR2 mRNA in the hippocampus of the injured side increased significantly after 3 to 21 days of brain injury, and the CCL2 mRNA in the hippocampus decreased significantly after hyperbaric oxygen treatment. Compared with TBI group, there was a significant difference between 14 d group and 21 d group in TBI group. CCR2 mRNA in hippocampus decreased significantly after hyperbaric oxygen treatment for 7 d and 14 d after hyperbaric oxygen therapy. Compared with TBI group, there was a significant difference between 7 d group and 14 day group (P 0.05). Conclusion the treatment of TBI can improve the cognitive function of traumatic brain injury rats, and its mechanism may be related to the down-regulation of CCL2/CCR2 expression in hippocampus.
【作者单位】： 南通大学附属医院;
【分类号】：R651.15
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本文编号：1625507