水下火器爆炸致兔急性闭合性肝损伤的实验研究

发布时间：2018-03-20 06:53

本文选题：急性闭合性肝损伤　切入点：爆炸伤　出处：《安徽医科大学学报》2017年02期 　论文类型：期刊论文

【摘要】：目的探讨水下火器爆炸致兔急性闭合性肝损伤的病理生理机制。方法选取30只兔,随机分为空白对照组(10只)和实验组(20只),实施水下爆炸损伤腹部,制作急性闭合性肝损伤模型,分别于爆炸损伤12、24 h后采样(每时间点各10只),测定肝湿/干重比(W/D)、动脉血氧分压(Pa O2)、谷丙转氨酶(ALT)和炎性因子。选取肝组织,显微镜观察光镜下肝组织病理形态。结果光镜下,实验组12 h起可见肝组织片状出血,肝血管破裂出血明显,肝组织内见片状坏死,肝索破坏,炎细胞浸润,偶见肝细胞嗜酸性变。与空白对照组比较,实验组W/D明显升高(P0.05),Pa O2明显降低(P0.05)。与空白对照组比较,实验组ALT指数明显升高(P0.05),炎性因子较空白对照组明显升高(P0.05)。结论水下爆炸冲击波可导致实验兔急性闭合性肝损伤,引起相应病理生理学改变。
[Abstract]:Objective to investigate the pathophysiological mechanism of acute closed liver injury induced by underwater firearm explosion in rabbits. Methods 30 rabbits were randomly divided into control group (n = 10) and experimental group (n = 20). A model of acute closed liver injury was established. The liver wet / dry weight ratio (W / D), arterial partial pressure of oxygen (Pao _ 2), alanine aminotransferase (alt) and inflammatory factors were measured at 12 h after explosion injury (n = 10) and inflammatory factors, respectively. Results under the light microscope, the experimental group showed the liver tissue lamellar hemorrhage, the rupture of the hepatic vessels, the necrosis of the liver tissue, the destruction of the hepatic cord and the infiltration of inflammatory cells. Compared with the blank control group, compared with the blank control group, the W / D of the experimental group was significantly higher than that of the blank control group, and the P / D of the experimental group was significantly higher than that of the control group, and that of the experimental group was significantly lower than that of the blank control group. The ALT index of the experimental group was significantly higher than that of the control group, and the inflammatory factor was significantly higher than that of the blank control group. Conclusion the underwater blast wave can cause acute closed liver injury and the corresponding pathophysiological changes in the experimental rabbits.
【作者单位】：安徽医科大学第一附属医院普通外科;中国人民解放军第一二三医院肝胆外科;中国科学技术大学近代力学系;中国人民解放军杭州疗养院全军创伤康复中心;
【基金】：南京军区科技创新课题面上项目(编号:15MS150)
【分类号】：R657.3

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