骨髓间充质干细胞对失血性休克大鼠早期炎性反应的影响

发布时间：2018-03-21 21:47

本文选题：骨髓间充质干细胞　切入点：失血性休克　出处：《中国老年学杂志》2016年05期 　论文类型：期刊论文

【摘要】：目的探讨骨髓间充质干细胞对失血性休克大鼠血清炎性因子及重要脏器的影响。方法体外分离培养大鼠骨髓间充质干细胞。雄性SD大鼠随机分为假手术组、模型组、治疗组(n=20)。改良Wiggers法制备大鼠出血性休克模型,休克持续90 min。治疗组在复苏前给予106个第3代骨髓间充质干细胞磷酸盐缓冲液(PBS)悬液1 ml,假手术组和模型组给予相同体积PBS溶液。分别于复苏后6、12、24、48、72 h取血清,酶联免疫试验(ELISA)检测肿瘤坏死因子(TNF)-α,白介素(IL)-1β、IL-6和IL-10水平,苏木素-伊红(HE)染色观察心、肝、肺、肾病理损伤。结果治疗组血清TNF-α和IL-6在复苏后12~72 h内较模型组显著下降(P0.05);治疗组血清IL-1β在复苏后6~12 h内较模型组下降显著(P0.05);治疗组IL-10水平在复苏后6~48 h内均较模型组有显著升高(P0.05)。骨髓间充质干细胞减轻肺、肝、肾、心等器官的炎性细胞浸润。结论骨髓间充质干细胞能调节失血性休克早期重要炎性因子水平,促进机体促炎-抗炎系统平衡。
[Abstract]:Objective to investigate the effects of bone marrow mesenchymal stem cells (BMSCs) on serum inflammatory factors and important organs in rats with hemorrhagic shock. Methods Rat bone marrow mesenchymal stem cells were isolated and cultured in vitro. Male SD rats were randomly divided into sham operation group and model group. The model of hemorrhagic shock was established by modified Wiggers method in the treatment group. Shock lasted 90 mins. The treatment group was given 1 ml of phosphate buffer solution of bone marrow mesenchymal stem cells (BMSCs) before resuscitation, and the sham-operated group and model group were given the same volume of PBS solution. Enzyme-linked immunosorbent assay (Elisa) was used to detect the levels of TNF- 伪, IL-1- 尾 -IL-6 and IL-10, and hematoxylin-eosin (HEH) staining to observe the heart, liver and lung. Results the levels of serum TNF- 伪 and IL-6 in the treatment group were significantly lower than those in the model group within 1272 h after resuscitation, while the serum IL-1 尾 in the treatment group was significantly lower than that in the model group within 6h after resuscitation (P 0.05), and the IL-10 level in the treatment group was higher than that in the model group within 648 h after resuscitation. Group A had a significant increase in P0.05. Bone marrow mesenchymal stem cells (BMSCs) alleviated the lung. Conclusion Bone marrow mesenchymal stem cells can regulate the level of important inflammatory factors in the early stage of hemorrhagic shock and promote the balance of pro-inflammatory and anti-inflammatory system.
【作者单位】：吉林大学口腔医院;
【基金】：吉林省产业技术研究与开发项目(吉发改高技〔2013〕779号)
【分类号】：R459.7

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