辽西地区汉族人群ABCA1基因R219K基因多态性与急性心肌梗死的相关性分析

发布时间：2018-03-26 13:28

本文选题：急性心肌梗死　切入点：三磷酸腺苷结合盒转运子　出处：《辽宁医学院》2013年硕士论文

【摘要】：目的研究三磷酸腺苷结合盒转运子A1基因R219K多态性与辽西地区汉族人群急性心肌梗死易感性及血脂水平的相关性。方法本研究选用辽西地区汉族人群作为研究对象，急性心肌梗死患者103例，正常对照114例。使用聚合酶链反应-限制性片段长度多态性技术对217例样本进行基因分型，描述ABCA1基因R219K多态性基因型分布特征，，分析该位点与急性心肌梗死易感性的关联。同时采用生化技术检测研究对象的血脂水平，分析不同基因型与血脂水平的关系。结果 1、ABCA1基因R219K多态性存在于辽西地区汉族人群中，该位点多态性有三种:RR型、RK型和KK型。在健康人群中RR型占29.8%，RK型占47.4%，KK型占22.8%。在急性心肌梗死组中RR型占43.7%，RK型占45.6%，KK型占10.7%。 2、ABCA1基因R219K多态性三种基因型频率在急性心肌梗死组和对照组中分布差异有统计学意义(P0.05)；K等位基因在急性心肌梗死组和对照组中分布差异有统计学意义（P0.01）。 3、健康对照组中K等位基因携带者高密度脂蛋白胆固醇水平高于非K携带者，分布差异有统计学意义(P0.05)。对照组中甘油三酯、总胆固醇、低密度脂蛋白胆固醇水平在基因型亚组间的差异无统计学意义(P0.05)。结论 ABCA1基因R219K位点多态性与急性心肌梗死有相关性；K等位基因可能是急性心肌梗死的保护基因。K等位基因携带者血浆高密度脂蛋白胆固醇水平高于非K携带者。
[Abstract]:Purpose. To study the relationship between R219K polymorphism of adenosine triphosphate binding cassette transporter A1 gene and susceptibility to acute myocardial infarction (AMI) and blood lipid level in Han population in western Liaoning province. Method. In this study, 103 patients with acute myocardial infarction (AMI) and 114 normal controls were enrolled in this study. 217 samples were genotyped by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique. The distribution characteristics of R219K polymorphism genotype of ABCA1 gene were described, and the association between R219K polymorphism and susceptibility to acute myocardial infarction was analyzed. At the same time, the blood lipid level was detected by biochemical technique, and the relationship between different genotypes and serum lipid level was analyzed. Results. 1the R219K polymorphism of ABCA1 gene was found in Han population in western Liaoning, and there were three types of RK and KK in this locus. In the healthy population, the RR type was 29.8and the RK type was 47.4%. In the acute myocardial infarction group, the RR type was 43.7RK type and 45.6KK type, 10.7% in the acute myocardial infarction group. 2There was significant difference in the distribution of R219K polymorphism of ABCA1 gene between the acute myocardial infarction group and the control group. There was significant difference in the distribution of P0.05T allele between the acute myocardial infarction group and the control group. 3. The level of high density lipoprotein cholesterol in K allele carriers in healthy control group was higher than that in non K carriers, and the distribution difference was statistically significant (P 0.05). There was no significant difference in low density lipoprotein cholesterol level among genotypic subgroups (P 0.05). Conclusion. The polymorphism of R219K locus of ABCA1 gene is associated with acute myocardial infarction. It may be that the plasma high density lipoprotein cholesterol level in patients with acute myocardial infarction is higher than that in non-K carriers.
【学位授予单位】：辽宁医学院
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R542.22

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