右美托咪定对脓毒血症大鼠肾功能及紧密连接蛋白表达的影响

发布时间：2018-03-28 16:07

本文选题：右美托咪定　切入点：脓毒血症　出处：《中国比较医学杂志》2016年04期

【摘要】：目的探讨右美托咪定对脓毒血症大鼠肾功能及紧密连接蛋白表达的影响。方法 SD大鼠30只,随机分为右美托咪定处理组(Dex组)、脓毒血症模型组(M组)、生理盐水对照组(C组),每组10只。Dex组大鼠静脉注射脂多糖(LPS)5 mg/kg,之后泵注Dex 7μg/kg,15 min后调整剂量为5μg/kg·h,持续泵注30 min;模型组按同样方法给予LPS后泵注生理盐水;C组始终采用上述方法给予生理盐水。24 h后取血液分离血浆,多功能生化仪检测肌酐(Scr)和尿素氮(BUN)含量;ELISA检测IL-1β和TNF-α含量;Western blot检测肾组织中紧密连接蛋白ZO-1和occludin的表达变化。结果与C组相比,模型组损伤较重,可见大量炎性细胞浸润,有肾小球肾炎表现,而Dex组未见明显的炎症细胞,细胞异常较少,与模型组相比,损伤减轻。肾功能及炎性因子检测结果显示,与C组比较,各组血浆中Scr、BUN、IL-1β和TNF-α含量均显著升高(P0.05),ZO-1和occludin蛋白表达水平均下降(P0.05);与模型组比较,Dex组各项指标均明显降低(P0.05),ZO-1和occludin蛋白表达水平均升高(P0.05)。结论右美托咪定可改善脓毒血症所致的急性肾损伤的肾功能,抑制炎症反应,提升紧密连接蛋白,对肾脏有一定的保护作用。
[Abstract]:Objective to investigate the effect of dexmedetomidine on renal function in rats with sepsis and the expression of tight junction protein. Methods 30 SD rats were randomly divided into dexmedetomidine group (Dex group), sepsis model group (M group), saline control group (C group), 10 rats in each group.Dex group of rats by intravenous injection of lipopolysaccharide (LPS) after infusion of Dex 5 mg/kg, 7 g/kg, 15 min after adjusting the dose of 5 g/kg / h, continuous infusion of 30 min; model group were given LPS after infusion of normal saline in the same way; C group always adopts the method given saline.24 after h the blood plasma separation, multifunctional biochemical detection of serum creatinine (Scr) and urea nitrogen (BUN) content; ELISA detection of IL-1 beta and TNF- alpha content; the expression of tight junction protein ZO-1 and occludin detection of Western blot in renal tissue. Results compared with C group, the model group had more severe injury, there are a lot of inflammatory cells infiltration of glomerular nephritis table Now, while the Dex group had no obvious inflammatory cells, abnormal cells less, compared with the model group. Reduce the damage of renal function and inflammatory factor test results showed that compared with C group, plasma Scr, BUN, IL-1 beta and TNF- alpha were significantly increased (P0.05), the expression level of ZO-1 and occludin protein decreased (P0.05); compared with the model group, the indexes of group Dex were significantly lower (P0.05), the expression level of ZO-1 and occludin protein were increased (P0.05). Conclusion dexmedetomidine can improve sepsis induced acute kidney injury renal function, inhibit inflammatory reaction, enhance the tight junction protein, protection effects on the kidney.

【作者单位】：延边大学临床学院;沈阳军区总医院心内科;吉林大学第一医院肾病科;
【分类号】：R459.7

【相似文献】