脓毒症患者外周血调节性Vδ1T细胞比例及功能变化

发布时间：2018-03-29 00:37

本文选题：脓毒症　切入点：外周血　出处：《南京医科大学学报(自然科学版)》2016年01期

【摘要】：目的:观察脓毒症患者外周血Vδ1 T细胞数量和功能的改变,初步探讨Vδ1 T细胞在脓毒症发病中的作用。方法 :选取2013年12月—2014年12月在本院ICU入住的脓毒症患者40例及同期入院进行健康体检者(health control,HC)40例,清晨空腹抽取外周静脉血10 m L。采用流式细胞术测定其外周血Vδ1 T细胞比例及Vδ1 T细胞表面Foxp3表达情况,采用CFSE染色方法检测Vδ1 T细胞对CD4+T细胞增殖能力的影响,采用流式细胞术测定Vδ1 T细胞与CD4+T细胞共孵育后对CD4+T细胞分泌γ-干扰素(interferonγ,IFN-γ)及肿瘤坏死因子α(tumor necrosis factorα,TNF-α)能力的影响。结果 :脓毒症患者外周血Vδ1 T细胞数量(3.35±0.55)%与HC(1.04±0.22)%相比显著升高(P0.01)。与HC外周血Vδ1 T细胞表面Foxp3表达水平(10.02±2.31)%相比,脓毒症患者外周血Vδ1 T细胞表面Foxp3表达水平(16.83±3.63)%显著升高(P0.01)。HC外周血Vδ1 T细胞对CD4+T细胞增殖、分泌IFN-γ和TNF-κ的抑制百分率分别为(44.26±6.31)%、(34.84±4.83)%和(39.31±4.91)%,脓毒症患者外周血Vδ1 T细胞对CD4+T细胞增殖、分泌IFN-γ和TNF-κ的抑制百分率分别为(62.35±5.91)%、(50.37±4.77)%和(54.94±5.71)%。提示,脓毒症外周血Vδ1 T细胞较HC外周血Vδ1 T细胞具有更强的免疫抑制功能(P0.01)。结论:脓毒症患者外周血Vδ1 T细胞免疫抑制功能增强,致使脓毒症患者免疫功能受到抑制,脓毒症患者免疫功能的这种改变可能与脓毒症的发生相关。
[Abstract]:Objective: to observe the changes of the number and function of V 未 1 T cells in peripheral blood of patients with sepsis. To explore the role of V 未 1 T cells in the pathogenesis of sepsis. Methods: 40 patients with sepsis admitted in our hospital from December 2013 to December 2014 and 40 patients who were admitted to our hospital at the same time were enrolled in this study. In the morning, 10 mL of peripheral venous blood was drawn on an empty stomach. The percentage of V 未 1 T cells and the expression of Foxp3 on the surface of V 未 1 T cells were measured by flow cytometry. The effect of V 未 1 T cells on the proliferation of CD4 T cells was detected by CFSE staining. The effects of V 未 1 T cells co-incubated with CD4 T cells on the ability of CD4 T cells to secrete interferon 纬 -interferon 纬 (IFN- 纬) and tumor necrosis factor 伪 -tumor necrosis factor 伪 (TNF- 伪) were determined by flow cytometry. Results: the number of V 未 1 T cells in peripheral blood of patients with sepsis was 3.35 卤0.55% and 0.55% respectively. HC(1.04 卤0.22% significantly increased the level of Foxp3 expression on the surface of V 未 1 T cells in the peripheral blood of HC, 10. 02 卤2. 31%, compared with 10. 02 卤2. 31% of the HC peripheral blood V 未 1 T cells. The expression of Foxp3 on the surface of V 未 1 T cells in patients with sepsis was significantly increased by 16.83 卤3.63%. The inhibitory rates of V 未 1 T cells on the proliferation of CD4 T cells and the secretion of IFN- 纬 and TNF- 魏 were 44.26 卤6.31 卤34.84 卤4.83% and 39.31 卤4.91%, respectively. The proliferation of CD4 T cells by V 未 1 T cells in peripheral blood of patients with sepsis was significantly higher than that in patients with sepsis, and the expression of IFN- 纬 and TNF- 魏 in peripheral blood of patients with sepsis was 34.84 卤4.83% and 39.31 卤4.91%, respectively. The inhibitory rates of IFN- 纬 and TNF- 魏 were 62.35 卤5.91% and 54.94 卤5.71%, respectively. The results suggest that V 未 1 T cells in peripheral blood of sepsis have stronger immunosuppressive function than those of HC peripheral blood V 未 1 T cells. Conclusion: the immunosuppressive function of V 未 1 T cells in peripheral blood of septic patients is enhanced. As a result, the immune function of sepsis patients is inhibited, and the change of immune function may be related to the occurrence of sepsis.
【作者单位】：新疆医科大学附属中医院重症医学科;
【分类号】：R459.7

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