大鼠肝脏炎症环境下肝卵圆细胞增殖模型的建立及评价

发布时间：2018-04-03 03:21

本文选题：肝炎症环境　切入点：肝卵圆细胞　出处：《石河子大学》2017年硕士论文

【摘要】：目的:建立肝脏炎症环境下肝卵圆细胞(HOC)的增殖模型,初步观察肝脏炎症环境下肝卵圆细胞的部分干细胞生物学特性方法:将55只雄性SD大鼠随机分为正常对照组、慢性肝炎模型组和肝炎+2-AAF模型组、2-AAF/PH模型组。采用CCL4复合法制备慢性肝炎动物模型;以CCL4复合法为基础,灌胃2-乙酰氨基芴(2-AAF)制备炎症环境下肝卵圆细胞增殖模型;肝2/3切除并术后灌胃2-AAF制备非炎症环境下HOC增殖模型。HE染色及VG胶原染色观察肝病理组织学改变,免疫组织化学法观察EPCAM和Thy-1的表达。结果:正常对照组大鼠肝组织中未见肝卵圆细胞;肝炎症模型组可见少量的肝卵圆细胞增殖;肝炎症+2-AAF模型组可见明显的肝卵圆细胞增殖,其中以8周的肝组织中最显著。与2-AAF/PH非炎症模型组比较,肝炎症环境中肝卵圆细胞高表达Thy-1。结论:CCL4复合法结合2-AAF灌胃可构建较为稳定的炎症环境下HOC增殖模型;炎症环境是HOC表现出肝癌干细胞部分生物学特性的原因之一。
[Abstract]:Objective: to establish the proliferative model of hepatic oval cell (Hoca) in the environment of liver inflammation, and to observe the biological characteristics of some stem cells in hepatic oval cells. Methods: 55 male Sprague-Dawley rats were randomly divided into normal control group.Chronic hepatitis model group and hepatitis 2-AAF model group 2-AAFP-PH model group.The animal model of chronic hepatitis was established by CCL4 compound method, and the proliferation model of hepatic oval cells was established by intragastric administration of 2-acetylaminofluorene (2-AAFF) on the basis of CCL4 compound method.HOC proliferation model was established by 2 / 3 resection of liver and intragastric administration of 2-AAF after operation. He staining and VG collagen staining were used to observe the histopathological changes of liver and the expression of EPCAM and Thy-1 were observed by immunohistochemical method.Results: no hepatic oval cells were found in the normal control group, a small number of hepatic oval cells proliferated in the liver inflammation model group, and obvious hepatic oval cell proliferation was observed in the 2-AAF model group, especially in the liver tissue at 8 weeks.The expression of Thy-1 in hepatic oval cells was higher than that in 2-AAF/PH non-inflammatory model group.Conclusion the HOC proliferation model can be constructed by the combination of 2-AAF with the combined method of 10% CCL4, and the inflammatory environment is one of the reasons why HOC shows some biological characteristics of HCC stem cells.
【学位授予单位】：石河子大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R575.3

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