创伤性脑损伤合并骨折中加速骨折愈合过程中相关因子的表达

发布时间：2018-04-04 06:02

本文选题：组织工程　切入点：神经生长因子　出处：《中国组织工程研究》2017年32期

【摘要】：背景:颅脑损伤能促进骨折愈合的现象,近年逐渐成为热点话题,但其发生机制还不明确。目的:建立颅脑损伤合并右侧胫骨骨折兔模型,观察血清及脑脊液降钙素基因相关肽及神经生长因子及胰岛素样生长因子1在骨折愈合中的变化趋势。方法:将新西兰大白兔随机分为4组,对照组、单纯颅脑创伤组、单纯骨折组及颅脑创伤合并骨折组,后3组分别建立颅脑损伤模型、右侧胫骨骨折模型及颅脑损伤合并右侧胫骨骨折模型,对照组为正常新西兰大白兔。建模后检测各组不同时间脑脊液、血清中的3种因子表达水平。对于骨折组及颅脑创伤合并骨折组分别建模后1,4,8周麻醉处死,取骨全长拍X射线片,测量骨痂体积并进行病理分析。结果与结论:颅脑创伤合并骨折组骨折愈合速度比单纯骨折组快;建模后4周颅脑创伤合并骨折组骨痂体积、骨小梁宽度、骨小梁面积比、新生血管数量明显高于单纯骨折组(P0.05);颅脑创伤合并骨折组血清及脑脊液中3种因子较单纯骨折组明显升高(P0.05),且3种因子于脑脊液中达峰时间较血清明显提前。在颅脑创伤合并骨折组中3种因子相比,其中降钙素基因相关肽升高时间较神经生长因子、胰岛素样生长因子1早。结果证实,在颅脑损伤后促进骨折愈合过程中,降钙素基因相关肽、神经生长因子及胰岛素样生长因子1是促进骨折愈合的相关因素,其中降钙素基因相关肽促进骨折愈合的速度最快。
[Abstract]:Background: craniocerebral injury can promote fracture healing, which has become a hot topic in recent years, but its mechanism is not clear.Aim: to establish a rabbit model of craniocerebral injury with right tibial fracture and to observe the tendency of serum and cerebrospinal fluid calcitonin gene-related peptide, nerve growth factor and insulin-like growth factor 1 in fracture healing.Methods: new Zealand white rabbits were randomly divided into 4 groups: the control group, the simple traumatic brain injury group, the simple fracture group and the craniocerebral trauma combined fracture group.Right tibia fracture model and craniocerebral injury combined with right tibial fracture model, the control group was normal New Zealand white rabbit.After modeling, the expression levels of three factors in cerebrospinal fluid (CSF) and serum at different time were detected.For the fracture group and the craniocerebral trauma combined with fracture group, the rats were anesthetized for 8 weeks after modeling, the full-length bone was taken for X-ray, the volume of callus was measured and the pathological analysis was made.Results and conclusion: the healing speed of craniocerebral trauma combined with fracture group was faster than that of simple fracture group, the volume of callus, trabecular width, trabecular area ratio of bone trabeculae, and the ratio of callus volume, trabecular width and trabecular area ratio of craniocerebral traumaThe number of neovascularization was significantly higher than that of simple fracture group (P 0.05), and the three factors in serum and cerebrospinal fluid (CSF) in TBI complicated fracture group were significantly higher than those in simple fracture group (P 0.05), and the peak time of these three factors in CSF was significantly earlier than that in serum.Calcitonin gene-related peptide increased earlier than nerve growth factor (NGF) and insulin-like growth factor 1 (IGF-1) in craniocerebral trauma with fracture group.The results showed that calcitonin gene-related peptide, nerve growth factor and insulin-like growth factor 1 were related factors in promoting fracture healing after craniocerebral injury.Calcitonin gene-related peptide promotes fracture healing fastest.
【作者单位】：新疆医科大学第一附属医院;
【基金】：新疆维吾尔自治区自然科学基金(2014211C045)~~
【分类号】：R651.15;R683

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