重组人粒细胞巨噬细胞集落刺激因子对脓毒症模型小鼠的治疗作用

发布时间：2018-04-10 07:55

本文选题：粒细胞巨噬细胞集落刺激因子　切入点：重组　出处：《中国组织工程研究》2016年49期

【摘要】：背景:研究发现粒细胞集落刺激因子可以用于治疗粒细胞减少症,关于粒细胞集落刺激因子治疗脓毒症的研究不多。目的:探讨重组人粒细胞巨噬细胞集落刺激因子对脓毒症小鼠的治疗作用。方法:采用腹腔内注射脂多糖的方法建立脓毒症小鼠动物模型,建模后6,30 h皮下注射重组人粒细胞巨噬细胞集落刺激因子,观察小鼠的肺组织病理变化、外周血中性粒细胞CD64表达以及血清肿瘤坏死因子α、白细胞介素10水平。结果与结论:(1)治疗后小鼠支气管上皮尚完整,间质轻度增生,见少量中性粒细胞浸润;(2)治疗后1,3,7 d治疗组小鼠外周血中性粒细胞CD64的表达和血清白细胞介素10水平显著高于模型组和正常对照组(P0.05);(3)治疗后1,3,7 d治疗组小鼠血清肿瘤坏死因子α水平明显高于正常对照组(P0.05),但低于模型组(P0.05);(4)结果表明,重组人粒细胞巨噬细胞集落刺激因子具有增强脓毒症小鼠中性粒细胞功能和抗炎作用。
[Abstract]:Background: it has been found that granulocyte colony stimulating factor can be used to treat granulocytopenia, but few studies have been done on the treatment of sepsis with granulocyte colony stimulating factor.Aim: to investigate the therapeutic effect of recombinant human granulocyte macrophage colony stimulating factor (RHGF) on sepsis mice.Methods: an animal model of sepsis was established by intraperitoneal injection of lipopolysaccharide. Recombinant human granulocyte macrophage colony stimulating factor was injected subcutaneously at 6h after modeling and the pathological changes of lung tissue were observed.The expression of CD64 in peripheral blood neutrophils and the levels of tumor necrosis factor 伪 and interleukin 10 in serum.Results and conclusion: after treatment, the bronchial epithelium of mice was intact and the interstitial hyperplasia was mild.The expression of CD64 in peripheral blood neutrophils and the level of serum interleukin 10 in the treatment group were significantly higher than those in the model group and the normal control group on the 7th day after treatment.The level of tumor necrosis factor 伪 was significantly higher than that of the normal control group (P 0.05), but lower than that of the model group (P 0.05).Recombinant human granulocyte macrophage colony stimulating factor can enhance neutrophil function and anti-inflammatory effect in septic mice.
【作者单位】：郑州大学第一附属医院ICU;
【基金】：河南省高等学校重点科研项目计划(15A320065)~~
【分类号】：R459.7;R-332

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