活化蛋白C对大鼠重症急性胰腺炎相关肾损害的保护作用研究

发布时间：2018-04-12 21:37

本文选题：活化蛋白C + SAP　；参考：《昆明医科大学》2014年硕士论文

【摘要】：[目的] 探讨活化蛋白C (actived protein C APC)对重症急性胰腺炎(SAP)相关肾损害的治疗作用及机制。 [方法] 健康成年雌鼠Sprague-Dawley (SD)大鼠随机分成四组：①假手术组(5只)；②SAP组(5只)；③治疗对照组(5只)；④治疗组(8只)。假手术组：随机取5只作为假手术组大鼠,开腹后轻轻翻动胰腺和十二指肠后关腹；SAP组、治疗对照组、治疗组：随机取大鼠多只,用5%牛磺胆酸钠(1ml/kg)进行胰胆管逆行注射,诱导SAP模型后5小时以大鼠内眦静脉血测血清肌酐值筛选出SAP并发肾功能损伤(肌酐大于98μmol/1)的大鼠18只,取其中5只作为SAP组大鼠；再取5只并经尾静脉注射生理盐水0.5ml作为治疗对照组；最后剩余大鼠8只,将相应大鼠治疗量的APC (50μg/kg)溶解于0.5ml生理盐水中后,注射到8只大鼠尾静脉中作为治疗组大鼠。四组动物在完成假手术或者诱导SAP模型后16个小时处死,收集血液,胰腺组织和肾组织标本。血液标本用于测定血清淀粉酶(AMY),Cr(肌酐)和BUN(血尿素氮)以及血清肿瘤坏死因子-a(TNF-a)和白介素-8(IL-8)水平(ELISA法),胰腺组织和肾组织标本用于病理切片进行病理学观察并评分。 [结果] 1治疗组血清AMY水平明显低于治疗对照组(P0.01)；SAP组该指标明显高于假手术组(P0.01)。 2治疗组胰腺组织损伤病理学评分、肾组织损伤病理学评分明显低于治疗对照组(P0.01)；SAP组该两种指标均明显高于假手术组(P0.01)。 3治疗组TNF-α、IL-8、Cr、BUN水平均低于治疗对照组(P0.05)；SAP组该四种指标均明显高于假手术组(P0.01)。 [结论] 活化蛋白C可降低IL-8与TNF-α水平及炎症反应,对重症急性胰腺炎相关肾损害具有保护作用。
[Abstract]:[purpose]To investigate the therapeutic effect and mechanism of activated protein C APC on renal damage associated with severe acute pancreatitis (SAP).[methods]Healthy adult female rats (Sprague-Dawley) were randomly divided into four groups: one sham-operated group (n = 5), a sham-operated group (n = 5), a control group (n = 5), a control group (n = 5) and a control group (n = 5).At 5 hours after induction of SAP model, 18 SAP rats complicated with renal function damage (creatinine > 98 渭 mol / 1) were selected from the serum creatinine values of the inner canthus vein of rats. 5 of them were taken as SAP group rats.Five rats were injected with saline 0.5ml via caudal vein as the control group, and the remaining 8 rats were treated with the corresponding dosage of APC 50 渭 g / kg, then injected into the tail vein of 8 rats as the treatment group.The animals in the four groups were killed 16 hours after sham-operation or SAP model. Blood, pancreas and kidney were collected.Blood samples were used to measure serum amylase (Cr) and bun (blood urea nitrogen), serum tumor necrosis factor (TNF- 伪) and interleukin-8 (IL-8) levels were determined by Elisa. Pancreatic and renal specimens were used for pathological observation and grading.[results]1the serum AMY level in the treatment group was significantly lower than that in the control group (P 0.01) and was significantly higher than that in the sham operation group (P 0.01).2the pathological scores of pancreatic injury and renal injury in the treatment group were significantly lower than those in the control group (P 0.01) and SAP group (P 0.01), which were significantly higher than those in the sham operation group (P 0.01).(3) the levels of TNF- 伪 IL-8Cr bun in the treatment group were lower than those in the control group (P 0.05) and SAP group were significantly higher than those in the sham operation group (P 0.01).[conclusion]Activated protein C can reduce the levels of IL-8 and TNF- 伪 and the inflammatory response, and has protective effect on renal damage associated with severe acute pancreatitis.
【学位授予单位】：昆明医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R657.51

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