大黄素甲醚联合脐带间充质干细胞移植治疗大鼠创伤性脑损伤的实验研究

发布时间：2018-04-14 18:07

本文选题：人脐带间充质干细胞 + 移植　；参考：《兰州大学》2013年硕士论文

【摘要】：目的通过研究大黄素甲醚对人脐带间充质干细胞(hUC-MSCs)植入创伤性脑损伤(TBI)大鼠体内后,基质金属蛋白酶和特异性神经元以及凋亡基因表达的影响,探讨大黄素甲醚联合hUC-MSCs移植治疗颅脑损伤的可行性依据。方法ELISA法检测基质金属蛋白酶-9(MMP-9)和金属蛋白酶组织抑制-1(TIMP-1)的表达。免疫组织化学法检测神经元特异性烯醇化酶(NSE)、胶质纤维酸性蛋白(GFAP)的表达；RealtimePCR检测凋亡因子Bcl-2和Bax的表达。结果与模型组比较,hUC-MSCs移植组、联合组大鼠脑组织中MMP-9表达减弱,TIMP-1表达增强；神经元特异性烯醇化酶(NSE)、胶质纤维酸性蛋白(GFAP)的表达增强, bcl-2基因的表达显著,bax基因的表达减弱,联合组上述表达更为显著。结论①hUC-MSCs移植入TBI大鼠脑组织后能够存活,并在大鼠脑组织中移行、分化为神经细胞,可表达神经元特异性烯醇化酶(NSE)、胶质纤维酸性蛋白(GFAP);大黄素甲醚组表达更为显著。②大黄素甲醚与hUC-MSCs对维护BBB的完整性有协同作用③大黄素甲醚可明显减少脑损伤区凋亡基因水平的表达作用。
[Abstract]:Objective to study the effects of emodin methyl ether on the expression of matrix metalloproteinases, specific neurons and apoptotic genes in injured brain injury (TBI) rats after implantation of human umbilical cord mesenchymal stem cells (hUC-MSCs).To investigate the feasibility of emodin methyl ether combined with hUC-MSCs transplantation in the treatment of craniocerebral injury.Methods ELISA assay was used to detect the expression of matrix metalloproteinase-9 (MMP-9) and tissue inhibitor of metalloproteinase-1 (TIMP-1).The expression of neuron-specific enolase (NSE) and glial fibrillary acidic protein (GFAP) were detected by immunohistochemistry. The expression of apoptotic factor Bcl-2 and Bax was detected by Realtime PCR.Results compared with the model group, the expression of MMP-9 decreased and TIMP-1 increased, the expression of neuron-specific enolase (NSEP) and glial fibrillary acidic protein (GFAP) increased, and the expression of bcl-2 gene decreased significantly in the combined group.The above expression was more significant in the combined group.Conclusion 1hUC-MSCs can survive in the brain tissue of TBI rats and differentiate into nerve cells.The expression of neuron-specific enolase (NSE) and glial fibrillary acidic protein (GFAP) in the emodin methyl ether group was more significant than that in the emodin methyl ether group. 2. The synergistic effect of emodin methyl ether and hUC-MSCs on maintaining the integrity of BBB. 3 emodin methyl ether could significantly reduce brain damage.Expression of apoptotic gene in injured area.
【学位授予单位】：兰州大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R651.15

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