miR-16在金黄色葡萄球菌脓毒症中的表达及意义探讨

发布时间：2018-04-15 14:36

  本文选题：miR- + 金黄色葡萄球菌　； 参考：《中国人兽共患病学报》2017年05期

【摘要】：目的探讨miR-16表达水平与金黄色葡萄球菌脓毒症严重程度的关联性,进一步探讨其潜在的临床意义。方法收集金黄色葡萄球菌脓毒症血液标本共计32例,脓毒性休克、严重脓毒症和一般脓毒症各8例,不同年龄段的健康对照24例;另外,收集革兰氏阴性菌脓毒症血液标本8例。用Trizol液裂解全血后提取microRNA,采用荧光定量PCR测定miR-16在不同组的表达水平(2-△△Ct法),用SPSS 13.0软件分析各组之间的统计学差异。采用SPSS 13.0软件将miR-16定量值与相应CRP和PCT值进行相关性分析。结果 miR-16表达水平与金黄色葡萄球菌脓毒症严重程度呈明显的负关联,各实验组与对照组相比均有统计学差异(P0.001),实验组之间也有统计学差异(P0.01)。miR-16表达水平与CRP和PCT值呈负相关性,相关系数分别为-0.561和-0.769。结论 miR-16表达与金黄色葡萄球菌脓毒症有明显的负关联性,提示其可能作为该菌脓毒症严重程度的标记物。
[Abstract]:Objective to investigate the relationship between the expression of miR-16 and the severity of Staphylococcus aureus sepsis and its potential clinical significance.Methods A total of 32 blood samples of Staphylococcus aureus sepsis were collected, including 8 cases of septic shock, 8 cases of severe sepsis and 24 cases of normal sepsis.Blood samples of Gram-negative bacterial sepsis were collected from 8 cases.MicroRNAs were extracted from whole blood with Trizol solution. The expression level of miR-16 in different groups was determined by fluorescence quantitative PCR. The statistical difference was analyzed by SPSS 13.0 software.SPSS 13.0 software was used to analyze the correlation between the quantitative value of miR-16 and the corresponding CRP and PCT values.Results there was a significant negative correlation between the expression of miR-16 and the severity of Staphylococcus aureus sepsis. There was a significant difference between the experimental group and the control group (P 0.001). There was also a negative correlation between the expression of miR-16 and CRP and PCT between the experimental group and the control group.The correlation coefficients were -0.561 and -0.769, respectively.Conclusion there is a negative correlation between miR-16 expression and Staphylococcus aureus sepsis, which suggests that miR-16 may be a marker for the severity of sepsis of Staphylococcus aureus.
【作者单位】： 杭州医学院检验医学院;浙江省台州医院特需病区;杭州医学院基础医学部;浙江省人民医院临床检验中心;
【基金】：浙江省自然科学基金(LY16H190006) 国家自然科学青年基金(31501581) 浙江省教育厅一般科研项目(Y201534394) 浙江医学高等专科学校科学研究计划项目(2015XZA02)~~
【分类号】：R459.7
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