香烟烟雾对大鼠创面愈合的影响

发布时间：2018-04-19 13:59

本文选题：香烟烟雾 + 创面愈合　；参考：《山西医科大学》2013年硕士论文

【摘要】：目的观察香烟烟雾对大鼠创面愈合时间的影响；及对各组固定时间创面愈合率的影响；CD68单抗标记免疫组化法计数各组创面愈合过程中巨噬细胞数量,探讨巨噬细胞对创面愈合的可能影响。方法选取健康成年SD大鼠40只,体重190-260g,随机分为4组：对照组(C组)、低焦油含量组(L组)、中焦油含量组(M组)和高焦油含量组(H组),每组10只。每组各只大鼠背部皮肤脱毛后画出2cm直径的4个圆圈,按画定的圆圈剪掉皮肤全层,形成4个等大的圆形创面。各创面间距大于2cm。C组不做任何干预,L组、M组、H组分别将大鼠置于自制的熏烟箱(30L)给予O.lmg、0.5mg、0.8mg不同等级焦油含量的香烟进行烟熏,每天30分钟,直到创面愈合。观察创面愈合时间,并于第7天及16天观察创面愈合率各组分别与创面愈合的第3天、第7天取创面周围组织,通过免疫组化法,单抗标记CD68+,统计巨噬细胞数量[1]。结果创面愈合时间：高(H组)、中(M)组、低(L组)剂量组愈合时间明显长于对照组(C),具有统计学意义(P0.05)。提示焦油含量高、中、低浓度组均有延长创面愈合时间的作用。高、中焦油含量浓度组较低焦油含量组更能延长愈合时间。创面愈合率：与对照组(C组)相比,高(H组)、中(M组)、低(L组)焦油含量组第7、16天创面愈合率均低于对照组组,具有统计学意义(P0.05),其中高(H组)中(M组)剂量组创面愈合率明显低于低(L组)剂量组(P0.05)。巨噬细胞数量：高(H)、中(M)、低(L)剂量组第7天创面肉芽组织巨噬细胞数量明显少于对照组(C)。结论香烟烟雾导致创面愈合延迟,固定时间创面愈合率降低,创面肉芽组织巨噬细胞数量减少。
[Abstract]:PurposeTo observe the effect of cigarette smoke on wound healing time of rats and the effect of fixed time wound healing rate in each group the number of macrophages in wound healing process was counted by immunohistochemical method with CD68 monoclonal antibody.To explore the possible effects of macrophages on wound healing.MethodForty healthy adult SD rats, weighing 190-260 g, were randomly divided into 4 groups: control group (C), low tar content group (L group), middle tar content group (M group) and high tar content group (H group) with 10 rats in each group.In each group, 4 circles with 2cm diameter were drawn after hair removal on the back skin of each group. The whole skin layer was cut out according to the circle drawn, and 4 equal size circular wounds were formed.The distance of each wound was larger than that of 2cm.C group without any intervention. The rats in group H were placed in a self-made fumigation box (30L). The rats were fumigated with 0. 5 mg 0. 5 mg of 0. 5 mg of 0. 8 mg of tar and smoked for 30 minutes every day until the wound healed.The wound healing time was observed, and the wound healing rate was observed on the 7th and 16th days, respectively. The tissue around the wound was taken on the 3rd day and the 7th day, and the number of macrophages was counted by immunohistochemical method and CD68 labeled by monoclonal antibody.ResultWound healing time: the healing time of the high dose group and the low dose group was significantly longer than that of the control group (P 0.05).It was suggested that the high, medium and low concentration of tar could prolong the healing time of wound.The healing time of high and middle tar concentration group was longer than that of low tar content group.Wound healing rate: compared with the control group (C group), the wound healing rate of the group with high Tar content, group M, and group L) was lower than that of the control group on day 716, and the rate of wound healing was lower than that of the control group on day 716.The wound healing rate in group M was significantly lower than that in group L (P 0.05).Number of macrophages: the number of macrophages in the granulation tissue of the high dose group was significantly lower than that of the control group on the 7th day.Conclusion cigarette smoke can delay wound healing, decrease wound healing rate and decrease the number of granulation macrophages.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R641

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