Tβ4基因对大鼠急性心肌梗塞后心功能的影响

发布时间：2018-04-21 05:39

本文选题：胸腺素β4 + 心肌梗死　；参考：《华中科技大学》2013年硕士论文

【摘要】：目的探讨慢病毒介导的胸腺肽β4(Thymosinbeta4Tβ4)基因经梗死区直接注射对大鼠急性心肌梗塞后心功能的影响。方法雄性Sprague-Dawley(SD)大鼠24只，体重200-220g，随机平均分为三组，即A.假手术组(sham组)：冠脉不结扎；B.LAD结扎组+经心肌表面注入等量生理盐水（NS组）；C.LAD结扎组+经心肌表面注入携带Tβ4的慢病毒载体（Tβ4组）。4周后，心脏B超测定大鼠的心功能，取梗死区心肌组织，TUNEL法检测细胞凋亡情况，，Westernblot法检测Tβ4蛋白和VEGF蛋白的表达，RT-PCR法检测梗死区心肌Tβ4mRNA的表达。结果4周后，Tβ4组与sham组相比心功能无明显差异，Tβ4组与NS组相比，EF%值明显改善（P0.01）,Tβ4组心肌细胞凋亡较NS组明显减少（P0.01），Tβ4组与NS组、sham组相比，Tβ4蛋白的表达上调（P0.05），VEGF作为血管新生的指标，Tβ4组较NS组、sham组比较其表达也有明显上调（P0.05）。Tβ4组mRNA的表达较其它两组也增多（P0.05）。结论经梗死区直接注射慢病毒载体携带的Tβ4基因可以改善心梗后心功能。
[Abstract]:Objective to investigate the effect of lentivirus-mediated thymosin 尾 4(Thymosinbeta4T 尾 4 gene injection into infarcted area on cardiac function after acute myocardial infarction in rats. Methods Twenty-four male Sprague-Dawley SD rats, weighing 200-220 g, were randomly divided into three groups: A. Sham group: coronary artery ligation group without coronary artery ligation B.LAD ligation group was injected with the same amount of normal saline on the surface of myocardium. The cardiac function of rats was measured by echocardiography after 4 weeks of injection of the lentivirus carrier T 尾 4 carrying T 尾 4 on the surface of the myocardium in the NS group and C. LAD ligation group. The expression of T 尾 4 protein and VEGF protein were detected by Western blot and the expression of T 尾 4mRNA was detected by reverse transcription-polymerase chain reaction (RT-PCR). Results after 4 weeks, there was no significant difference in cardiac function between T 尾 4 group and sham group. Compared with NS group, T 尾 4 group significantly improved the cardiac myocyte apoptosis of T 尾 4 group compared with NS group. Compared with NS group, cardiac myocyte apoptosis in T 尾 4 group decreased significantly compared with that in NS group, and the expression of T 尾 4 protein in T 尾 4 group increased the expression of T 尾 4 protein as a vascular vessel. The expression of mRNA in T 尾 4 group was significantly higher than that in NS group. The expression of mRNA in T 尾 4 group was significantly higher than that in the other two groups. Conclusion the T 尾 4 gene carried by lentivirus vector directly injected into the infarct area can improve the cardiac function after myocardial infarction.
【学位授予单位】：华中科技大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R542.22

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