垂体中叶素联合降钙素原在急性胰腺炎患者血清中的动态改变及临床意义

发布时间：2018-04-24 16:41

  本文选题：垂体中叶素 + IMD　； 参考：《青岛大学》2015年硕士论文

【摘要】：目的:早期检测不同严重程度胰腺炎大鼠血清垂体中叶素(Intermedin,IMD)含量,探究血清IMD对急性胰腺炎大鼠的早期预测作用。方法:SD大鼠120只,随机分成4组:其中对照手术组(NS,n=30)、轻度急性胰腺炎组(mild acute pancreatitis,MAP,n=30)、重症急性胰腺炎组(severe acute pancreatitis,SAP,n=30)、SAP+乌司他丁干预组别(SAP+Ulinastatin,UTI,n=30)。急性胰腺炎大鼠造模成功后分别在3小时、6小时和12小时处死而分别采集血样和胰腺组织,运用ELISA法检测血清IMD含量、胰腺组织进行病理学评分和免疫组化定性检测胰腺组织IMD表达情况。结果:MAP组、SAP组和SAP+Ulinastatin组血清中IMD含量显著降低于NS组,差异具有统计学意义(F=2.062,P0.05):MAP组胰腺炎大鼠血清IMD含量比SAP组仅轻度增加,SAP+Ulinastatin组比SAP组血清IMD含量轻度增加;MAP组、SAP组和SAP+Ulinastatin组随着时间的延长血清IMD含量降低;MAP组、SAP组和SAP+Ulinastatin组病理学评分各组间差异显著,随着严重程度的加重而评分增加,具有统计学意义(F=14.152,P0.05);各组胰腺炎大鼠组织病理学评分随着时间的延长而增加。MAP组别3小时、6小时和12小时血清IMD含量与胰腺炎组织病理学评分间存在负相关(r3=-0.637,P0.05r6=-0.375,P0.05;r12=0.261,P0.05);SAP组别3小时、6小时和12小时血清IMD含量与胰腺炎组织病理学评分间存在负相关(r3=-0.52,P0.05 r6=-0.17,P0.05;r12=-0.03,P0.05).大鼠胰腺组织免疫组化结果显示大鼠胰腺组织中存在IMD的表达。结论:IMD对胰腺组织可能具有保护重要作用,早期检测血清IMD对大鼠胰腺炎诊断具有重要价值。目的:探究垂体中叶素联合降钙素原在急性胰腺炎患者血清中的动态改变及临床意义。方法:根据急性胰腺炎诊断指南,将急性胰腺炎患者分为轻症胰腺炎组(MA P组,n=60)及重症胰腺炎(SAP组,n=30)。检测MAP组和SAP组患者在入院24小时、48小时、72小时以及第7天时血清IMD和PCT含量,同时患者入院24小时内进行APACHEⅡ评分;将同时间的健康体检人群作为正常对照组(NS组,n=30);比较血清IMD和PCT的改变以及差异性。将血清IMD和PCT、IMD和APACHEⅡ评分以及PCT和APACHEⅡ评分进行相关性分析。结果:MAP组和SAP组PCT随着时间的推移,PCT含量逐渐升高,差异显著(P0.05),SAP组PCT含量明显高于MAP组;MAP组和SAP组IMD含量随着时间的推移,IMD含量逐渐降低,差异显著(P),SAP组IMD含量明显低于MAP组,差异显著(p0.05);血清IMD含量与PCT呈现负相关,对急性胰腺诊断具有一定价值;MAP组和SAP组血清IMD含量与APACHEⅡ评分均呈负相关(rs0,p0.05).MAP组和SAP组血清PCT含量与APACHEⅡ评分均呈正相关关(rs0,p0.05)。结论:血清IMD、PCT可以作为早期诊断急性胰腺炎的指标,对重症胰腺炎的诊断也具有一定价值。
[Abstract]:Aim: to investigate the early predictive effect of serum IMD on rats with acute pancreatitis by detecting the content of intermedin in serum of rats with acute pancreatitis. Methods 120 small Sprague-Dawley rats were randomly divided into four groups: control group (n = 30), mild acute pancreatitis group (n = 30) and severe acute pancreatitis group (n = 30). Rats with acute pancreatitis were killed at 3 hours, 6 hours and 12 hours after successful modeling, and blood samples and pancreatic tissues were collected respectively. Serum IMD levels were measured by ELISA method. The expression of IMD in pancreatic tissues was detected by pathological score and immunohistochemical staining. Results the content of IMD in serum of SAP group and SAP Ulinastatin group was significantly lower than that of NS group. The difference was statistically significant (P < 0.05). The serum IMD content of rats in SAP group was only slightly higher than that in SAP group. The serum IMD content in SAP Ulinastatin group was slightly higher than that in SAP group. The serum IMD content in map group and SAP Ulinastatin group decreased with the prolongation of time. There was significant difference between the pathological score of SAP Ulinastatin group and that of SAP Ulinastatin group. As the severity increases, the score increases, The histopathological score of rats with pancreatitis increased with time. There was a negative correlation between the serum IMD content of 3 hours and 12 hours in map group and the histopathological score of pancreatitis. There was a negative correlation between the serum IMD content and the histopathological score of pancreatitis group. There was a negative correlation between the serum IMD content and the histopathological score of pancreatitis at 3 hours, 6 hours and 12 hours, respectively, and there was a negative correlation between the levels of serum IMD and the histopathological score of pancreatitis. Immunohistochemical results showed that there was IMD expression in pancreatic tissue of rats. Conclusion the early detection of serum IMD may play an important role in the protection of pancreatic tissue, and early detection of serum IMD may be of great value in the diagnosis of pancreatitis in rats. Objective: to investigate the dynamic changes and clinical significance of pituitary mesophylloin combined with procalcitonin in serum of patients with acute pancreatitis. Methods: according to the diagnostic guidelines of acute pancreatitis, the patients with acute pancreatitis were divided into mild pancreatitis group (MA P group) and severe pancreatitis group (SAP group). The serum levels of IMD and PCT were measured in MAP group and SAP group at 24 hours, 48 hours, 72 hours and 7 days after admission, and APACHE 鈪，

本文编号：1797454