硫酸锌联合阿司匹林对大鼠急性心肌梗死的保护作用及机制研究

发布时间：2018-04-26 02:20

  本文选题：心肌梗死 + 硫酸锌　； 参考：《重庆医科大学学报》2016年12期

【摘要】：目的:探讨硫酸锌联合阿司匹林治疗对大鼠急性心肌梗死的疗效及其机制。方法:选取100只200 g左右的雌性SD大鼠,随机分为5组,每组20只。假手术组不结扎并按常规饮食喂养,其余4组结扎并在常规饮食的基础上分别给以无菌蒸馏水(对照组)、硫酸锌、阿司匹林、硫酸锌联合阿司匹林,连续给药7 d后,使用超声心动图及侵入性导管检测心功能及血流动力学指标,并对大鼠血液中的肌钙蛋白T进行检测,然后取出心脏切片进行HE和Masson染色检验,检测各组心脏组织中的转化生长因子-β1(transforming growth factor-β1,TGF-β1)、环氧合酶-2(cyclooxygenase-2,COX-2)、超氧化物歧化酶1(superoxide dismutase-1,SOD-1)和谷胱甘肽过氧化物酶-4(glutathione peroxidase-4,GPX-4)的m RNA和蛋白质的表达情况。结果:给以阿司匹林和硫酸锌联合阿司匹林均能改善心肌梗死情况,保护受损心肌;与单独使用阿司匹林比较,硫酸锌联合阿司匹林更能显著提高收缩压[(119±11)mm Hg vs.(103±7)mm Hg,P=0.037]、舒张压[(98±4)mm Hg vs.(83±6)mm Hg,P=0.041]、平均动脉压[(103±6)mm Hg vs.(89±5)mm Hg,P=0.029]及Emax[(5.27±0.33)mm Hg/μL vs.(4.13±0.29)mm Hg/μL,P=0.027];降低炎性侵润、心肌梗死面积、心率[(328±11)beats/min vs.(364±19)beats/min,P=0.016]、全身血管阻力[(6.70±0.39)mm Hg×min/μL vs.(8.73±0.59)mm Hg×min/μL,P=0.039]及心肌梗死标志物肌钙蛋白T的水平[(583±51)pg/m L vs.(819±57)pg/m L,P=0.017];同时也更能降低促炎细胞因子TGF-β1和COX-2的m RNA[(0.65±0.18)vs.(1.48±0.12),P=0.014;(0.70±0.12)vs.(1.21±0.08),P=0.031]及蛋白[(2.42±0.19)vs.(3.61±0.27),P=0.032;(1.76±0.32)vs.(5.20±0.59),P=0.018)]表达水平,增加抗氧化酶SOD-1和GPX-4的m RNA[(2.14±0.11)vs.(1.58±0.12),P=0.021;(2.69±0.17)vs.(1.87±0.07),P=0.018]及蛋白[(5.40±0.62)vs.(2.40±0.33),P=0.036;(4.26±0.47)vs.(2.72±0.51),P=0.023]表达水平。结论:硫酸锌联合阿司匹林可有效保护急性心肌梗死对心肌的损伤,其主要的作用机制可能是通过抑制促炎细胞因子TGF-β1和COX-2,激活抗氧化酶类SOD-1和GPX-4的水平,实现抗炎抗氧化的作用,从而达到对急性心肌梗死的保护效果。
[Abstract]:Objective: to investigate the effect and mechanism of zinc sulfate combined with aspirin on acute myocardial infarction in rats. Methods: 100 female SD rats of about 200 g were randomly divided into 5 groups with 20 rats in each group. The sham-operation group was not ligated and fed on a conventional diet, while the other four groups were given aseptic distilled water (control group, zinc sulfate, aspirin, zinc sulfate combined with aspirin for 7 days). Cardiac function and hemodynamics were measured by echocardiography and invasive catheters. Cardiac troponin T was detected in the blood of rats. The heart sections were taken out for HE and Masson staining. The expression of m RNA and protein of transforming growth factor 尾 1(transforming growth factor- 尾 1 (TGF- 尾 1), cyclooxygenase-2cyclooxygenase-2 (COX-2), superoxide dismutase-1 (SOD-1) and glutathione peroxidase-4GPX-4 (Glutathione peroxidase 4GPX-4) in heart tissues were detected. Results: aspirin and zinc sulfate combined with aspirin could improve myocardial infarction and protect damaged myocardium, compared with aspirin alone. Zinc sulfate combined with aspirin could significantly increase systolic blood pressure [119 卤11)mm Hg vs.(103 卤7)mm P0. 037], diastolic blood pressure [98 卤4)mm Hg vs.(83 卤6)mm HgP0. 041], mean arterial pressure [103 卤6)mm Hg vs.(89 卤5)mm HgP0. 029] and Emax [5. 27 卤0.33)mm Hg/ 渭 L vs.(4.13 卤0.29)mm Hg/ 渭 L P0. 027]. 蹇冪巼[(328卤11)beats/min vs.(364卤19)beats/min,P=0.016],鍏ㄨ韩琛，

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