枯否细胞通过组织因子对急性胰腺炎大鼠凝血系统变化的影响

发布时间：2018-04-27 01:15

本文选题：枯否细胞 + 组织因子　；参考：《重庆医科大学》2014年硕士论文

【摘要】：目的：观察大鼠急性胰腺炎组织因子(TF)及凝血系统的变化，探讨枯否细胞(KCs)通过组织因子对大鼠急性胰腺炎(AP）凝血系统的影响。方法：108只SD大鼠完全随机分为假手术(SO)组、轻型胰腺炎(MAP)组、重型胰腺炎(SAP)组、SO+氯化钆(Gdcl3)预处理组、MAP+Gdcl3组、SAP+Gdcl3组。Gdcl3预处理组在诱发AP模型前24h予尾静脉注射Gdcl3(10mg／kg，0.1mL)，分别用1.5%和5%的牛黄胆酸钠胰胆管逆行注射诱导MAP和SAP模型。建模后或假手术组分别于术后6、12、24h处死大鼠，检测其血清中淀粉酶及凝血象的变化；ELISA法检测血清中TF的含量；分离并培养KCs，采用Western Blot法检测其TF的蛋白表达量；免疫组化检测肝脏中TF的表达；病理切片观察胰腺的损伤程度。结果：大鼠血清中淀粉酶含量MAP组（2842±310IU/L）和SAP组（5973±452IU/L）明显高于SO组（1028±53IU/L）（p＜0.01），但与MAP+Gdcl3组（2749±352IU/L）、SAP+Gdcl3组（5868±483IU/L）比较无显著差异（p0.05）。血清中TF含量MAP组（10.453±0.225ng/l）和SAP组（14.472±1.623ng/l）较SO组（8.153±1.083ng/l）显著升高，MAP+Gdcl3组（8.851±1.211ng/L）和SAP+Gdcl3组（12.018±1.343ng/l）较MAP组和SAP组明显降低（p＜0.05）。MAP和SAP组大鼠肝脏中TF蛋白表达较SO组表达明显增加。MAP+Gdcl3和SAP+Gdcl3组KCs中TF蛋白含量较MAP组和SAP组明显降低（p＜0.05）。大鼠血清凝血酶原时间（PT）SAP组（22.05±0.94s）较MAP（13.42±0.57s）和SO组（13.62±0.61s）明显升高，SAP+Gdcl3组（18.91±0.78s）较SAP明显降低（p＜0.05）；大鼠血清活化部分凝血活酶时间（APTT）SAP组（36.13±2.85s）较MAP（18.26±2.20s）和SO组（18.13±2.23s）明显升高，SAP+Gdcl3组（31.86±2.53s）较SAP明显降低（p＜0.05）；大鼠血清纤维蛋白原浓度（FIB）SAP组（3.56±0.28g/l）较MAP（2.31±0.25g/l）和SO组（2.23±0.25g/l）明显升高，SAP+Gdcl3组（3.01±0.27g/l）较SAP明显降低（p＜0.05）。大鼠胰腺病理切片显示，MAP组胰腺间质水肿，MAP+Gdcl3组较MAP组病理损伤有所减轻；SAP组胰腺呈凝固性坏死，伴有大量炎症细胞浸润，SAP+Gdcl3组较SAP组病理损伤明显减轻。结论：AP时KCs中TF表达显著增加，，导致大鼠凝血时间延长；抑制KCs可有效降低AP时TF的表达，缩短大鼠凝血时间，减轻胰腺的组织病理损伤。
[Abstract]:Aim: to observe the changes of tissue factor (TFF) and coagulation system in acute pancreatitis (AP) rats, and to investigate the effect of Kupffer cells (KCs) on the coagulation system of acute pancreatitis (AP) in rats by tissue factor. Methods one hundred and eight Sprague-Dawley rats were randomly divided into two groups: sham operation group (SOgroup) and mild pancreatitis (MAPP) group. In SAP group, map Gdcl3 group and SAP Gdcl3 group 路Gdcl3 preconditioning group were injected with Gdcl310mg / kg 0.1mLL of Gdcl310mg / kg Gdcl3 respectively 24 hours before AP model. MAP and SAP models were induced by 1.5% and 5% sodium taurocholate retrograde injection into the pancreatopancreatic duct, respectively. After modeling or in sham operation group, rats were killed at 61224 hours after operation to detect the changes of serum amylase and coagulation, to detect the content of TF in serum by Elisa, to isolate and culture KCsand to detect the protein expression of TF by Western Blot method. The expression of TF in liver was detected by immunohistochemistry and the degree of pancreatic injury was observed by pathological section. Results: the content of amylase in serum of MAP group (2842 卤310 IUP / L) and SAP group (5973 卤452 IUU / L) was significantly higher than that of so group (1028 卤53IU/L)(p < 0.01), but there was no significant difference compared with that of MAP Gdcl3 group (2749 卤352IUP / L) and MAP Gdcl3 group (5868 卤483IUP / L). TF content in serum of MAP group (10.453 卤0.225 ng / l) and SAP group (14.472 卤1.623 ng / l) were significantly higher than that of so group (8.153 卤1.083 ng / L). Map Gdcl3 group and SAP Gdcl3 group were significantly lower than MAP group and SAP group in the expression of TF protein in liver. Map Gdcl3 and SAP Gdcl3 group KCs were significantly lower than those in MAP group and SAP group. The content of TF protein was significantly lower than that of MAP and SAP groups (P < 0.05). The serum prothrombin time of rats was 22.05 卤0.94s (compared with MAP(13.42 卤0.57s) and so group (13.62 卤0.61s). The serum prothrombin time of Gdcl3 group (18.91 卤0.78s) was significantly lower than that of SAP (P < 0.05), and the serum activated partial thromboplastin time of rats (36.13 卤2.85s vs MAP(18.26 卤2.20s) and so group 18.13 卤2.23s (18.13 卤2.23s) was significantly higher than that of SAP group (31.86 卤2.53s). The serum fibrinogen concentration in SAP group was 3.56 卤0.28 g / L, which was significantly higher than that in MAP(2.31 卤0.25 g / L group and so group (2.23 卤0.25 g / L). The serum fibrinogen concentration in Gdcl3 group was 3.01 卤0.27 g / L) significantly lower than that in SAP group (p < 0.05). The pathological sections of the rat pancreas showed that the pancreatic interstitial edema in the map group and the pathological damage in the map Gdcl3 group were less than those in the MAP group, and the pancreatic coagulation necrosis in the SAP group was alleviated, and the pathological damage in the SAP Gdcl3 group was significantly alleviated than that in the SAP group with a large number of inflammatory cells infiltrating. Conclusion the expression of TF in KCs increases significantly in the presence of 1: AP, which leads to the prolongation of coagulation time in rats, and the inhibition of KCs can effectively reduce the expression of TF in AP, shorten the coagulation time of rats, and alleviate the histopathological injury of pancreas.
【学位授予单位】：重庆医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R657.51

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