血清胱抑素C表达水平及其结构形式与急性脑缺血相关性分析

发布时间：2018-05-04 09:12

本文选题：急性脑缺血 + 胱抑素C　；参考：《宁夏医科大学》2017年硕士论文

【摘要】：目的:了解急性脑缺血患者血清胱抑素(Cys C)表达水平及其不同结构形式(单体/寡聚体)的变化;分析不同程度急性脑缺血(轻、中度)血清Cys C表达水平变化;为下一步研究Cys C不同结构形式(单体及寡聚体)对缺氧状态下神经细胞的增殖及凋亡的影响提供前期研究基础。方法:选取宁夏医科大学总医院神经内科及脑卒中病区自2016年5月1日-2016年8月31日收住院的急性脑梗死患者32例为病例组;根据入院当天NIHSS评分结果将病例组分为轻、中度。选取同期在本院体检中心体检的健康人群26例作为对照组。分别收集两组研究对象的临床资料,于空腹8小时后采血,用酶联免疫吸附实验(ELISA)法测定血清Cys C表达水平,并用western blotting法测定Cys C不同结构形式。结果:急性脑缺血组与对照组临床资料,性别、年龄、血压、血糖、吸烟、饮酒史进行比较,差异均无统计学意义(P0.05);急性脑缺血组患者血清Cys C水平[(1.086±0.658)mg/L]高于对照组[(0.687±0.099)mg/L],两组比较差异具有统计学意义(t=3.056,p=0.003);急性脑缺血组患者血清Cys C寡聚体率(87.5%)明显高于对照组(19.2%),两组相比差异有统计学意义(X2=27.261,P0.001)。比较分析不同程度急性脑缺血患者(轻、中度)Cys C表达水平,并通过Wilcoxon秩和检验提示两组间比较差异无统计学意义。结论:急性脑缺血患者血清Cys C表达水平升高;急性脑缺血患者血清Cys C表达水平升高是以寡聚体升高为主;本研究中急性脑缺血严重程度与Cys C表达水平无明显关系。
[Abstract]:Objective: to investigate the changes of serum cystatin C (Cys C) expression and its different structural forms (monomer / oligomer) in patients with acute cerebral ischemia, and to analyze the changes of serum Cys C expression in patients with acute cerebral ischemia (mild and moderate). It provides a basis for further study on the effects of different structural forms of Cys C (monomers and oligomers) on the proliferation and apoptosis of neurons under hypoxia. Methods: Thirty-two patients with acute cerebral infarction admitted from May 1, 2016 to August 31, 2016 in the Department of Neurology and Stroke in the General Hospital of Ningxia Medical University were selected as the case group, and the patients were divided into mild and moderate groups according to the NIHSS score on the day of admission. 26 healthy people were selected as control group. The clinical data of the two groups were collected respectively. Blood samples were collected 8 hours after fasting. The expression of Cys C in serum was determined by Elisa and the different structural forms of Cys C were determined by western blotting method. Results: the clinical data, sex, age, blood pressure, blood glucose, smoking and drinking history were compared between the acute cerebral ischemia group and the control group. The serum Cys C level in acute cerebral ischemia group [1.086 卤0.658)mg/L] was higher than that in control group [0.687 卤0.099)mg/L], the difference between the two groups was statistically significant, and the serum Cys C oligomer rate in acute cerebral ischemia group (87.5%) was significantly higher than that in control group (19.2g%). The difference between the two groups was statistically significant. The expression of Cys C in patients with different degrees of acute cerebral ischemia (mild and moderate) was compared and analyzed. The results of Wilcoxon rank sum test showed that there was no significant difference between the two groups. Conclusion: the expression of Cys C in the serum of patients with acute cerebral ischemia is higher than that of the patients with acute cerebral ischemia, and the level of Cys C in the patients with acute cerebral ischemia is mainly increased by oligodeoxynucleotides. There is no significant correlation between the severity of acute cerebral ischemia and the expression of Cys C in this study.
【学位授予单位】：宁夏医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R743.3

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