阻断白介素-3减轻脓毒血症小鼠的炎症反应

发布时间：2018-05-05 20:13

本文选题：脓毒血症 + 盲肠结扎穿孔手术　；参考：《中国现代医学杂志》2016年13期

【摘要】：目的探究阻断白介素-3(IL-3)对脓毒性小鼠炎症反应的作用及其潜在机制。方法小鼠盲肠结扎穿孔手术(CLP)后,分为野生型小鼠组和IL-3缺陷型小鼠组,每组13只,连续7 d观察和记录小鼠的死亡数。另取野生型小鼠和IL-3缺陷型小鼠,每组20只,分为3组进行实验:①组小鼠分别在CLP后0、12和24 h进行临床症状评分和体温测量;另在24 h测血压,采血,用于测定干扰素-α(IFN-α)、白介素-1β(IL-1β)和白介素-6(IL-6)水平;②组小鼠分别在CLP后0、6、12和24 h采血并在24 h取材(肝、肺),用于测定中性粒细胞、单核细胞总数及病理、免疫组织化学分析;③组小鼠分别在CLP后0和24 h采血并收集支气管肺泡灌洗液用于蛋白总量和生化指标[天冬氨酸转氨酶(AST)、丙氨酸转氨酶(ALT)]的测定。结果与野生型小鼠组比较,CLP后IL-3缺陷型小鼠的存活率和体温升高(P0.05),血压良好,临床症状评分下降(P0.05)。此外,与野生型小鼠组比较,CLP后IL-3缺陷型小鼠炎症细胞因子IFN-α、IL-1β和IL-6水平降低(P0.05);肺和肝组织中的中性粒细胞和单核细胞总数降低(P0.05);支气管肺泡灌洗液中的总蛋白和血生化指标(AST、ALT)也降低(P0.05)。结论阻断IL-3能够减轻脓毒性小鼠的炎症反应,提高脓毒性小鼠的存活率。
[Abstract]:Objective to explore the effect and potential mechanism of interleukin -3 (IL-3) on the inflammatory response of septic mice. Methods after the cecum ligation and perforation operation (CLP), the mice were divided into wild type mice and IL-3 deficient mice, with 13 rats in each group, and 7 d were observed and recorded in mice. The wild type and IL-3 deficient mice were taken from 20 mice in each group. The experiment was carried out in 3 groups: (1) the mice were tested for clinical symptoms and thermometer at 0,12 and 24 h after CLP, and the blood pressure was measured on 24 h, blood sampling was used to measure the level of interferon - alpha (IFN- -), interleukin -1 beta (IL-1 beta) and interleukin -6 (IL-6). Granulocyte, total number of mononuclear cells and pathological, immunohistochemical analysis; 3 groups of mice were collected at 0 and 24 h after CLP and collected bronchoalveolar lavage fluid for total protein and biochemical index [aspartate aminotransferase (AST), alanine aminotransferase (ALT)]. The results were compared with that of wild type mice and IL-3 deficient mice after CLP. In addition, the inflammatory cytokine IFN- alpha, IL-1 beta and IL-6 levels decreased (P0.05) in IL-3 deficient mice after CLP, and the total number of neutrophils and mononuclear cells in lung and liver tissues decreased (P0.05), and the total eggs of bronchoalveolar lavage fluid in the lung and liver tissues were compared with those in the wild type mice (P0.05). The AST (ALT) of white blood also decreased (P0.05). Conclusion blocking IL-3 can reduce the inflammatory reaction of septic mice and improve the survival rate of septic mice.

【作者单位】：福建医科大学附属泉州第一医院儿科;福建泉州医学高等专科学校;
【分类号】：R459.7

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