ERK通路阻滞剂PD98059对心肺复苏大鼠脑组织SOD表达和细胞凋亡的影响

发布时间：2018-05-07 12:34

本文选题：心脏骤停 + 心肺复苏　；参考：《山东医药》2016年38期

【摘要】：目的观察ERK通路阻滞剂PD98059(PD)对心肺复苏(CPR)后大鼠脑组织超氧化物歧化酶(SOD)表达以及脑细胞凋亡的影响。方法采用经食管交流电刺激诱导心室颤动建立大鼠心脏骤停/心肺复苏(CA/CPR)模型,将恢复自主循环(ROSC)的48只大鼠随机分成PD组和模型组各24只,分别静脉注射PD 0.3 mg/kg及等量生理盐水;选择6只大鼠为假手术组,只行手术操作不诱导CA/CPR。分别于ROSC后12、24、48、72 h时各处死6只大鼠,取脑组织;用免疫荧光标记法检测大脑皮层组织SOD1、SOD2荧光密度及Caspase3阳性细胞,TUNEL法检测凋亡细胞。结果与模型组比较,PD组各时点SOD1荧光密度均明显升高(P均0.05),PD组ROSC后12、24、48 h时SOD2荧光密度均明显升高(P均0.05)。ROSC后72 h,模型组、PD组Caspase3阳性表达率、脑细胞凋亡率均高于假手术组,而PD组Caspase3阳性表达率、脑细胞凋亡率低于模型组,P均0.05。结论 PD治疗能提高CA/CPR后模型大鼠脑组织SOD表达水平,抑制脑细胞凋亡。
[Abstract]:Objective to observe the effect of ERK pathway blocker PD98059 on the expression of superoxide dismutase (SOD) and apoptosis of brain cells after cardiopulmonary resuscitation (CPR) in rats. Methods the cardiac arrest / cardiopulmonary resuscitation (CAP / CPR) model was established in rats with ventricular fibrillation induced by transesophageal alternating current stimulation. 48 rats with spontaneous circulatory resuscitation were randomly divided into PD group and model group, 24 rats in each group. PD 0.3 mg/kg and saline were injected intravenously, and 6 rats were selected as sham-operated group. Six rats were killed at 12: 24 and 48 / 72 h after ROSC, respectively, and brain tissues were taken out, and the fluorescence density of SOD1 and SOD2 in cerebral cortex were detected by immunofluorescence labeling method and apoptotic cells were detected by Tunel method with Caspase3 positive cells. Results compared with the model group, the fluorescence density of SOD1 in PD group was significantly higher than that in PD group at all time points (P < 0.05). The fluorescence density of SOD2 in PD group was significantly higher than that in PD group at 48 h after ROSC. The positive expression rate of Caspase3 and the apoptosis rate of brain cells in model group were significantly higher than those in sham operation group. The positive expression rate of Caspase3 and apoptosis rate of brain cells in PD group were lower than those in model group (P < 0.05). Conclusion PD therapy can increase the expression of SOD and inhibit the apoptosis of brain cells after CA/CPR.
【作者单位】：广西医科大学;
【基金】：国家自然科学基金资助项目(81160231)
【分类号】：R459.7

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