轻度CLP模型大鼠在脓毒症早期即发生心肌收缩与舒张功能障碍

发布时间：2018-05-07 18:50

本文选题：脓毒症 + 心肌功能障碍　；参考：《中国病理生理杂志》2017年05期

【摘要】：目的:利用轻度盲肠结扎穿孔术(CLP)复制脓毒症大鼠模型,比较脓毒症大鼠心功能与其它器官功能障碍发生的时间。方法:将大鼠随机分为假手术组和盲肠结扎穿孔术组,分别于手术后6 h、9 h和12 h用Langendorff装置检测大鼠心肌收缩与舒张功能,并测定心肌肿瘤坏死因子α(TNF-α)、细胞间黏附分子1(ICAM-1)和血管内皮细胞黏附因子1(VCAM-1)的表达;同时检测血清TNF-α水平以及大鼠肝、肾功能和肺湿/干重比值。结果:轻度CLP术后10 d,大鼠的死亡率为26.7%。CLP术后9 h与12 h左心室内压最大上升与下降速率显著低于假手术组;CLP术后6 h,心肌TNF-α、ICAM-1和VCAM-1的mRNA表达显著高于假手术组,CLP术后9 h心肌TNF-α和VCAM-1的蛋白表达显著升高。CLP术后9 h血清天冬氨酸氨基转移酶活性和12 h丙氨酸氨基转移酶活性高于假手术组。而术后6 h、9 h和12 h,大鼠血尿素氮水平、肺湿/干重比与相应时点的假手术组比较,差异没有统计学显著性。结论:轻度CLP模型大鼠在脓毒症发生后6 h心肌炎症因子表达增多,9 h后即发生心肌内在收缩与舒张功能障碍,其发生早于肝、肾功能损伤。
[Abstract]:Aim: to establish sepsis rat model with mild cecal ligation and perforation, and compare the time between cardiac function and other organ dysfunction in septic rats. Methods: rats were randomly divided into sham operation group and cecal ligation and perforation group. Myocardial contractile and diastolic function were measured by Langendorff device at 6 h and 12 h after operation respectively. The expression of TNF- 伪, ICAM-1 and VCAM-1 were measured, and the levels of TNF- 伪 in serum, liver and kidney function and lung wet / dry weight ratio were also measured. Results: at 10 days after mild CLP, the death rate of rats was significantly lower than that of sham operation group at 9 and 12 hours after 26.7%.CLP. The mRNA expression of TNF- 伪 ICAM-1 and VCAM-1 in myocardium was significantly higher than that in sham operation group. The expression of TNF- 伪 and VCAM-1 in myocardium increased significantly at 9 h after operation. The activities of serum aspartate aminotransferase and alanine aminotransferase at 9 h after operation were significantly higher than those in sham operation group. However, there was no significant difference in blood urea nitrogen level and lung wet / dry weight ratio between sham operation group and sham operation group at 6 h and 12 h after operation. Conclusion: the expression of myocardial inflammatory factors in mild CLP model rats increased at 6 h after sepsis and 9 h after sepsis, the myocardial intrinsic systolic and diastolic dysfunction occurred, which occurred earlier than that of liver and kidney function injury.
【作者单位】：暨南大学基础医学院病理生理学系国家中医药管理局三级科研实验室;
【基金】：国家自然科学基金资助项目(No.81372028) 广东省教育厅学科建设科技创新项目(No.2013KJCX0019) 广州市科技计划项目(No.201508020005) 暨南大学高水平大学建设医学科学研究与应用组团联合研究项目(No.88016013045)
【分类号】：R-332;R459.7

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