TNF-α和IL-1β在急性呼吸窘迫综合征小鼠肺组织表达的变化及评价

发布时间：2018-05-08 08:12

本文选题：ARDS + TNF-α　；参考：《细胞与分子免疫学杂志》2017年02期

【摘要】：目的探究肿瘤坏死因子α(TNF-α)和白细胞介素1β(IL-1β)在急性呼吸窘迫综合征(ARDS)小鼠模型肺组织中表达水平随时间的变化规律,并评价其与小鼠肺组织损伤严重程度之间的相关性。方法采用脂多糖(LPS)诱导建立小鼠ARDS模型,HE染色观察小鼠肺组织形态结构改变,并计算肺损伤评分;实时定量PCR法检测小鼠肺组织内TNF-α和IL-1β的mRNA水平;ELISA检测小鼠支气管肺泡灌洗液(BALF)中TNF-α和IL-1β的含量。结果与正常对照组小鼠相比,ARDS小鼠的肺泡及间质组织正常结构破坏,炎症浸润明显,3 d时肺损伤评分达到最高值;ARDS小鼠肺组织中TNF-α和IL-1βmRNA表达水平显著升高,分别在LPS滴注后30 min和6 h达峰值;同时ARDS小鼠BALF中TNF-α和IL-1β含量也明显升高。结论 LPS诱导ARDS小鼠的肺组织损伤程度与TNF-α、IL-1β等炎症介质表达水平,随着时间表现出"驼峰样"升高,炎症介质的高值出现在肺损伤程度的高峰之前,提示这些炎症因子在ARDS炎症性损伤的形成及发展中扮演着重要角色。
[Abstract]:Objective to investigate the expression of tumor necrosis factor- 伪 (TNF- 伪) and interleukin-1 尾 (IL-1 尾) in lung tissue of acute respiratory distress syndrome (ARDS) mice, and to evaluate the relationship between the expression of TNF- 伪 and the severity of lung injury in mice with acute respiratory distress syndrome (ARDS). Methods the ARDS model was induced by lipopolysaccharide (LPS) in mice. The morphologic changes of lung tissue were observed by HE staining, and the lung injury score was calculated. The mRNA levels of TNF- 伪 and IL-1 尾 in the lung tissue of mice were detected by real-time quantitative PCR method. The content of TNF- 伪 and IL-1 尾 in bronchoalveolar lavage fluid (BALF) of mice was detected by enzyme-linked immunosorbent assay (Elisa). Results compared with the normal control group, the normal structure of alveolar and interstitial tissue in ARDS mice was destroyed, and the lung injury score reached the highest value at 3 days after obvious inflammatory infiltration. The expression levels of TNF- 伪 and IL-1 尾 mRNA in lung tissue of ARDS mice were significantly increased. The levels of TNF- 伪 and IL-1 尾 in BALF of ARDS mice were also significantly increased at 30 min and 6 h after infusion of LPS, and the contents of TNF- 伪 and IL-1 尾 in BALF of ARDS mice were also significantly increased. Conclusion the degree of lung injury induced by LPS and the expression of inflammatory mediators such as TNF- 伪 and IL-1 尾 in ARDS mice showed hump-like increase with time, and the high value of inflammatory mediators appeared before the peak of lung injury. These inflammatory factors play an important role in the formation and development of ARDS inflammatory injury.
【作者单位】：第四军医大学西京医院呼吸内科;
【基金】：国家自然科学基金(81570072)
【分类号】：R563.8

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